摘要
目的:研究长链非编码RNA-p23154(lncRNA-p23154)通过调节微小RNA375和Krüppel样因子4(KLF4)的表达对人卵巢癌细胞(SKOV3)增殖、侵袭能力的影响。方法:以SKOV3细胞为研究对象,通过转染实验,过表达(过表达组)与抑制(抑制组)细胞中lncRNA-p23154,并设置转染空质粒的SKOV3细胞为对照组,荧光实时定量聚合酶链式反应(qRT-PCR)检测转染效果;CCK8法及Tanswell实验检测各组SKOV3细胞增殖、侵袭情况;qRT-PCR、Western blot检测各组中miR-375及其靶基因KLF4的表达情况。结果:qRT-PCR显示SKOV3细胞中lncRNA-p23154过表达与抑制效果显著(均P<0.05);与过表达组相比,抑制组SKOV3细胞增殖和侵袭能力均升高(均P<0.05);qRT-PCR、Western blot检测发现,过表达组miR-375在mRNA水平低于抑制组、过表达组KLF4在mRNA和蛋白水平均高于抑制组(均P<0.05)。结论:lncRNA-p23154可能通过miR-375/KLF4途径,影响SKOV3细胞增殖、侵袭能力。
Objective:To observe the effect of long non-coding RNA(lncRNA)p23154 on the proliferation and invasion of ovarian cancer cells(SKOV3)by regulating miR-375 and Krüppel-like factor 4(KLF4).Methods:Over-expression(Over-expression group)and inhibition(inhibition group)of lncRNA p23154 in SKOV3 cells,and set up transfected cells with empty plasmid as control group(NC group).The qRT-PCR was used to detect the transfection effect.CCK8 method and Tanswell experiment were used to detect the proliferation and invasion of SKOV3 cells in each group.Western blot and qRT-PCR were used to detect the expression of miR-375 and its target genes KLF4 in each group.Results:The qRT-PCR showed that lncRNA p23154 overexpression and inhibition were significant(all P<0.05).The proliferation and invasion ability of SKOV3 cells in the inhibition group were both increased(all P<0.05).The level of miR-375 in the overexpression group was lower than that in the inhibition group,and KLF4 mRNA and protein levels in the overexpression group were higher than those in the inhibition group(all P<0.05).Conclusion:LncRNA p23154 may affect the proliferation and invasion of SKOV3 cells through the miR-375/KLF4 pathway.
作者
洪甲
杨洋
栾丽霞
HONG Jia;YANG Yang;LUAN Lixia(Department of Obstetrics,Xi’an International Medical Center Hospital,Xi’an 710100,China)
出处
《陕西医学杂志》
CAS
2021年第11期1336-1339,共4页
Shaanxi Medical Journal
基金
西安市科技计划项目[20YXYJ0005(6)]。
