摘要
选用不同基因类型的鲜食玉米(普甜玉米、超甜玉米、加强甜玉米和糯玉米)共15个材料作为母本,4个不同诱导材料作为父本,分别在景洪冬季和昆明夏季于抽丝后的不同时期进行杂交,统计诱导的单倍体后代,研究不同遗传背景、诱导基因、生态条件以及授粉时期对单倍体诱导率的影响。结果表明,不同的遗传背景、诱导基因、生态条件以及授粉时期对单倍体诱导均有重要影响,昆明夏季进行单倍体诱导的效率显著高于景洪冬季,吐丝早期授粉比晚期授粉的诱导效率要高;不同的母本基因型与不同的诱导系都对单倍体的诱导率产生极显著影响,但同一鲜食玉米类型不同品种的单倍体诱导率表现不同,说明单倍体诱导率的表达不受甜糯基因的影响;父本诱导系的诱导率则表现较为一致,都是诱导系CAU-1和(CAU-1×CAU-5)-B的单倍体诱导率显著高于诱导系(MT1×H780)-B和MT1的诱导率。在进行鲜食玉米单倍体育种时,选用诱导系CAU-1和(CAU-1×CAU-5)-B在昆明夏季于吐丝早期进行诱导,可以大幅提高单倍体的诱导率。
In the study, different genotypes of fresh maize(including normal sweet maize, super sweet maize,sugary enhanced maize, and waxy maize), a total of 15 materials as female and four different inducers as male were selected, the crosses were made during different pollination period in Jinghong winter season and Kunming summer season respectively, and the induced haploid offspring were statistical analyzed, finally tried to reveal the influence of different genetic background, different inducer genes, different ecological conditions and different pollination period on haploid inducing rate. The main results were as followed: different genetic background, inducer genes, ecological conditions and pollination periods had important influence on the haploid induction, the haploid inducing efficiency in Kunming summer season was significantly higher than that of in Jinghong winter season, pollination during early stage was significantly higher than that of in late stage, inducers CAU-1 and(CAU-1×CAU-5)-B was significantly higher than that of inducers(MT1×H780)-B and MT1. Therefore, it can be concluded that the haploid inducing rate can be greatly increased by using inducers CAU-1 and(CAU-1×CAU-5)-B, making pollination during early stage in Kunming summer season for fresh maize breeding.
作者
高佳琪
王琨
徐梅艳
毕文文
谭静
GAO Jia-qi;WANG Kun;XU Mei-yan;BI Wen-wen;TAN Jing(Resource Plant Research Institute,Yunnan University,Kunming 650500,China)
出处
《玉米科学》
CAS
CSCD
北大核心
2021年第5期15-21,27,共8页
Journal of Maize Sciences
基金
云南省应用基础研究计划重点项目(2015FA012)。
关键词
鲜食玉米
单倍体
诱导率
生态条件
遗传背景
授粉时期
Fresh maize
Haploid
Inducing rate
Ecological condition
Genetic background
Pollination period
