摘要
传统的数字全息显微技术通常采用高相干激光作为照明光源,虽然其全息图具有高的对比度,但是相干照明带来的相干噪声会降低再现像的信噪比。基于部分相干照明的数字全息显微技术(PCI-DHM),在保证相位测量精度的前提下,可以有效降低相干噪声。按照物光和参考光之间是否有夹角,PCI-DHM可以分为同轴相移和离轴PCI-DHM。介绍了同轴相移PCI-DHM和离轴PCI-DHM中的几种典型测量方案。同轴相移PCI-DHM采用等光程的物光和参考光形成同轴全息光路,利用相移干涉技术可以重建出样品的振幅/相位分布,利用光源的短相干特性,可以获得样品内部微观结构的层析图像。离轴PCI-DHM通常采用光栅在物光和参考光之间引入夹角,构成消色差干涉装置。离轴PCI-DHM技术可以通过记录单幅离轴全息图完成对动态样品振幅/相位分布的实时测量。最后,介绍了PCI-DHM在生物细胞、生物组织三维成像及微纳结构形貌测量等领域的应用。
The traditional digital holographic microscopy(DHM)usually adopts highly coherent lasers as an illumination source.Although the hologram has high contrast,the coherent noise induced by the coherent lasers reduces the signal-to-noise ratio of the reconstructed image.Partially coherent illumination-based DHM(PCI-DHM)can effectively reduce the coherent noise and maintain high phase-measurement accuracy.PCI-DHM can be divided into on-axis and off-axis PCI-DHMs depending on the interference angle between the object and reference waves.This study reviews the architectures of several typical PCI-DHMs and compares their advantages and disadvantages for different schemes.Specifically,on-axis phase-shifting PCI-DHM uses an on-axis optical configuration,where both the object and reference waves have the same optical path length,and amplitude/phase maps of the sample can be reconstructed using the phase-shifting technique.Moreover,a three-dimensional tomographic image of the internal microstructure in the sample can be obtained based on the short-coherence characteristic of light source.Off-axis PCI-DHM usually uses grating to introduce an angle between the object and reference waves,constructing an achromatic interferometer.The real-time measurement of amplitude/phase maps of the sample can be achieved only from an off-axis hologram.Finally,the practical applications of PCI-DHM in quantitative phase imaging of biological cells,biological tissue,microstructures,and nanostructures are introduced.
作者
王阳
张美玲
王宇
温凯
卓可群
郭荣礼
郜鹏
Wang Yang;Zhang Meiling;Wang Yu;Wen Kai;Zhuo Kequn;Guo Rongli;Gao Peng(School of Optoelectronic Engineering,Xi’an Technological University,Xi'an,Shaanxi,710021,China;School of Physics and Optoelectronic Engineering,Xidian University,Xi'an,Shaanxi,710071,China)
出处
《激光与光电子学进展》
CSCD
北大核心
2021年第18期141-156,共16页
Laser & Optoelectronics Progress
基金
国家自然科学基金(62075177,61975233)
陕西省自然科学基金(2020JM-193,2020JQ-324)
中央高校基本科研业务费专项资金(JB210513)
江西省图像处理与模式识别国家重点实验室基金(ET202080417)。
关键词
成像系统
数字全息显微
相移干涉
离轴干涉
部分相干照明
imaging systems
digital holographic microscopy
phase-shifting interference
off-axis interference
partially coherent illumination
