摘要
目的帕金森病(PD)纹状体神经元损伤是否牵涉到氧化应激因素一直备受关注,本文结合行为学分析探讨褪黑素(MT)对纹状体不同神经元活性氧自由基(ROS)反应的影响。方法随机将24只清洁级SD大鼠分为对照组、6OHDA组和6OHDA+MT处理组,实验借助行为学、组化、免疫组化技术对PD大鼠模型予以探察。结果(1)如同本实验室前期研究,6-羟基多巴胺(6OHDA)致使实验大鼠的学习记忆及肌张力改变,MT的使用明显改善其变化。(2)二氢乙锭(DHE)组化染色结果显示,作为氧化应激反应产物的ROS主要定位于纹状体投射神经元(NeuN+),同时可见一些单染色的ROS+以及NeuN+神经元。6OHDA组纹状体的ROS出现在NeuN+神经元的百分率(ROS-NeuN/ROS)以及NeuN+神经元呈现ROS+反应百分率(NeuN-ROS/NeuN)[分别为(80.34±1.75)%、(89.08±2.86)%]皆高于其对照组[分别为(68.67±2.57)%、(61.24±1.52)%](P<0.05),而使用MT明显遏制由6OHDA诱导升高的百分率[6OHDA+MT组分别为(71.78±3.19)%、(64.64±2.97)%](P<0.05)。(3)ROS-Parv双标记实验显示,作为纹状体主要类型的小清蛋白(Parv)中间神经元呈现ROS阳性反应,其百分率在6OHDA组(91.62±8.63)%高于它的对照组(68.66±7.52)%(P<0.05),MT对6OHDA诱导升高的百分率显示抑制效应(71.83±1.45)%(P<0.05)。结论本文结果提示氧化应激因素可能牵涉到PD纹状体神经元损伤的病理过程,而MT对其具有保护作用。
Objective Whether oxidative stress was involved in Parkinson’s disease(PD)striatal neuron damage has been a concern.This experiment combined behavioral analysis to explore the effect of melatonin(MT)on the reactive oxygen species(ROS)of different neurons in the striatum.Methods 24 clean SD rats were randomly divided into control group,60 HDA group and 60 HDA+MT treatment group.Present experiment used behavior,histochemistry,and immunohistochemical techniques to detect the PD rat model.Results(1)As in the previous research in this laboratory,60 HDA caused changes in the learning,memory and muscle tension of experimental rats,and the use of MT significantly improved the changes.(2)Dihydroethidium(DHE)staining results showed that ROS,which was the product of oxidative stress,was mainly located in striatal projection neurons(NeuN+),but some single-stained ROS+and NeuN+neurons can also be seen at the same time.Statistics showed that the percentage of ROS appearing in NeuN+neurons(ROS-NeuN/ROS)and the percentage of NeuN+neurons showing ROS+response(NeuN-ROS/NeuN)[(80.34±1.75)%,(89.08±2.86)%,respectively]in the striatum of the 60 HDA group were higher than those in the control group[(68.67±2.57)%,(61.24±1.52)%,respectively](P<0.05),and the use of MT significantly reduced the percentage of increase induced by 60 HDA[(71.78±3.19)%,(64.64±2.97)%,res pectively](P<0.05).(3)Furth er ROS-Parv double labeling experiment showed that Parvalbumin(Parv)interneurons,the main type of striatum,showed ROS positive reaction,and the percentage of striatum in 60 HDA group(91.62±8.63)%was higher than that of the control group(68.66±7.52)%(P<0.05).MT showed an inhibitory effect ROS increase induced by 60 HDA(71.83±1.45)%(P<0.05).Conclusion Present experimental results suggested that oxidative stress was involved in the PD pathological process of striatal neuron damage,and MT has a protective effect on it.
作者
贾林菊
陈思
黄子芸
朱耀峰
郑雪峰
陈涛
雷万龙
JIA Lin-ju;CHEN Si;HUANG Zi-yun;ZHU Yao-feng;ZHENG Xue-feng;CHEN Tao;LEI Wan-long(Department of Anatomy,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou 510080,China)
出处
《解剖学研究》
CAS
2021年第5期514-519,共6页
Anatomy Research
基金
国家自然科学基金地区科学基金项目(82160271)
贵州省科学技术基金黔科合基础-ZK[2021]一般414
湖南省中医药科研计划项目(2021086)
国家自然科学基金(81471288)。
关键词
帕金森病
纹状体
活性氧自由基
褪黑素
大鼠模型
Parkinson.s disease
Striatum
Reactive oxygen species
Melatonin
Rat model
