摘要
微生物在环境中无处不在,几乎占据了所有的栖息地。根据人体微生物组计划报道,健康成人体内90%的细胞都是微生物。那么,人死后微生物是否继续存在?会有怎样的变化?随着微生物组学和测序技术及人工智能技术的发展与成熟,国内外研究者们利用高通量测序技术发现,微生物群落会呈现出一定的演替规律,它们与死亡时间(postmortem interval,PMI)有一定的关联,同时利用人工智能技术结合测序后的微生物数据,能挖掘出有利于PMI推断的更深层的规律和有用的信息,这为PMI的推断打开了新的突破口。本文综述了近年来基于死后微生物群落变化的研究进展,揭示其在法医学PMI推断中的潜在应用价值。
Microbes are ubiquitous in the environment and occupy almost all habitats. According to the Human Microbiome Project, 90% of the cells inside healthy adults are microbes. So, do microbes still exist after human death? How will they change? With the development and maturity of microbiome, sequencing technologies and artificial intelligence, some researchers employed high-throughput sequencing technologies and discovered that the microbial community would show a certain succession with the occurrence of the decomposition of corpses, which was related to the postmortem interval(PMI)in forensic medicine. Moreover, artificial intelligence technology combined with the microbial sequencing data can be used to discover useful information and regularities for estimating PMI, opening a new gate for PMI estimation. In this paper, we reviewed recent advances in the study of postmortem succession of microbial community, aiming to demonstrate its potential application in forensic PMI estimation.
作者
王梓齐
张驰
康克莱
龚辰宇
郭立亮
季安全
聂胜洁
吴坚
王乐
Wang Ziqi;Zhang Chi;Kang Kelai;Gong Chenyu;Guo Liliang;Ji Anquan;Nie Shengjie;Wu Jian;Wang Le(National Engineering Laboratory for Forensic Science,Key Laboratory of Forensic Genetics of Ministry of Public Security,Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China;School of Forensic Medicine,Kunming Medical University,Yunnan,Kunming 650500,China)
出处
《中国法医学杂志》
CSCD
2021年第5期528-531,共4页
Chinese Journal of Forensic Medicine
基金
国家重点研发计划项目(2017YFC0803503)
公安部科技强警基础工作专项项目(2019GABJC15)
公安部物证鉴定中心基本科研业务费项目(2019JB009)。
关键词
微生物群落
高通量测序
死亡时间
人工智能技术
非人类DNA
Microbial community
High-throughput sequencing
Postmortem interval
Artificial intelligence technology
Non-Human DNA
