摘要
面对多种霉菌毒素的危害,依据本实验室建立的黄曲霉毒素B1(AFB1)、玉米赤霉烯酮(ZEA)和呕吐毒素(DON)联合导致的细胞毒性模型,通过复合益生菌、霉菌毒素降解酶及其配伍来降解这三种霉菌毒素。本研究首先选用拉丁方试验设计获得枯草芽孢杆菌、酿酒酵母菌和丁酸梭菌的最佳组合,然后再与霉菌毒素降解酶配伍。结果表明:当枯草芽孢杆菌、酿酒酵母菌和丁酸梭菌的活菌数皆为1×10^(5) cfu/mL时,对AFB1、ZEA和DON的联合降解效果最好。高含量霉菌毒素组AFB1、ZEA和DON的降解率分别为40.55%、56.05%和47.22%(P<0.05),低含量霉菌毒素组AFB1、ZEA和DON的降解率分别为43.05%、38.26%和80.49%(P<0.05)。复合益生菌与霉菌毒素降解酶的配伍试验结果表明,在高剂量霉菌毒素的降解试验中,单一复合益生菌、单一霉菌毒素降解酶及两者的配比为30:1时,对三种霉菌毒素的总降解率最高,分别到达了190.08%、175.10%和184.44%,显著高于其他组(P<0.05);在低剂量霉菌毒素的降解试验中,单一霉菌毒素降解酶对三种霉菌毒素的总降解率最高,达到了219.23%(P<0.05);其次为单一复合益生菌及两者的配伍比例为30:1和3:2时,对三种霉菌毒素的总降解率较高,分别达到了178.07%、181.84%和170.33%,显著高于其他组(P<0.05)。综上所述,复合益生菌、霉菌毒素降解酶及其两者适宜配伍,可同步降解AFB1、ZEA和DON三种霉菌毒素,为多种霉菌毒素的同步降解奠定了基础。
In order to degrade aflatoxin B_(1)(AFB_(1)),zearalenone(ZEA)and donxynivalenol(DON)synchronously based on the cytotoxicity models in our laboratory,Bacillus subtilis,Saccharomyces cerevisiae,Clostridium butyricum were selected and combined with mycotoxin-degradation enzymes.The optimal compound probiotics was obtained by Latin square design.The result showed that the synchronous degradation rates of AFB_(1),ZEA and DON were 40.55%,56.05%,47.22%in the high-cytotoxicity group(P<0.05),and 43.05%,38.26%,80.49%in the low-cytotoxicity group(P<0.05),when visible counts of Bacillus subtilis,Saccharomyces cerevisiae and Clostridium butyricum were 1×10^(5) cfu/mL,respectively.The effect of combination between compound probiotics and mycotoxin-degradation enzymes on AFB_(1),ZEA and DON degradation rates showed that total mycotoxin-degradation rates were 190.08%,175.10%and 184.44%by single compound probiotics,single mycotoxin-degradation enzymes,and both combination at a ratio of 30:1,which was higher than other groups(P<0.05)under high-level mycotoxin condition;total mycotoxin-degradation rate was 219.23%(P<0.05)by single mycotoxin-degradation enzymes,followed by 178.07%,181.84%and 170.33%by single compound probiotics,and both combination at a ratio of 30:1 and 3:2,which was higher than other groups(P<0.05)under low-level mycotoxin condition.In general,the single compound probiotics,single mycotoxin-degradation enzymes and optimal both combination provided an effective strategy for synchronous degradation of many kinds of mycotoxins.
作者
王晓敏
常娟
王平
尹清强
杨明凡
朱群
胡骁飞
王全亮
WANG Xiaomin;CHANG Juan;WANG Ping;YIN Qingqiang;YANG Mingfan;ZHU Qun;HU Xiaofei;WANG Quanliang(College of Animal Science and Technology,Henan Agricultural University,Zhengzhou,Henan Province 450046,China;College of Veterinary Medicine,Henan Agricultural University,Zhengzhou,Henan Province 450046,China;Henan Delin Biological Product Co.,Ltd.Xinxiang,Henan Province 453000,China;Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou,Henan Province 450002,China;Henan Guangan Biotechnology Co.,Ltd.Zhengzhou,Henan Province 450001,China)
出处
《中国饲料》
北大核心
2021年第21期85-91,共7页
China Feed
基金
河南省自然科学基金(182300410029)
河南省创新示范专项(201111311100)
新乡市重大科技专项(ZD19005)。
