摘要
目的探讨虎杖苷对脂多糖(LPS)诱导的皮肤成纤维细胞凋亡、氧化应激及炎性反应的保护作用。方法2021年1月至4月,以5 mg/L LPS刺激成纤维细胞建立实验模型,并随机分为4组:正常对照组细胞不接受任何处理;药物对照组细胞接受50μmol/L虎杖苷处理;实验组细胞接受5 mg/L LPS处理;治疗组细胞接受5 mg/L LPS及50μmol/L虎杖苷处理。细胞增殖检测试剂盒(CCK-8)检测细胞活力;末端脱氧核苷酸转移酶介导的dUTP缺口末端标记测定法[terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling,TUNEL]检测细胞凋亡;试剂盒检测细胞丙二醛(malonaldehyde,MDA)及超氧化物歧化酶(superoxide dismutase,SOD)含量;酶联免疫吸附剂测定(ELISA)检测培养基肿瘤坏死因子(TNF)-α及白介素(IL)-6含量。结果与正常对照组比较,实验组细胞凋亡率、MDA含量、培养基TNF-α及IL-6含量分别显著增加为(12.70±0.11)%、(3.38±0.29)nmol/(mg·pr)、(483.5±38.4)ng/L及(784.4±49.6)ng/L,细胞活力及SOD含量分别显著下降为(78.00±5.90)%及(11.5±1.3)U/(mg·pr)。与实验组比较,治疗组细胞凋亡率、MDA含量、培养基TNF-α及IL-6含量分别显著下降为(7.50±0.08)%、(2.18±0.19)nmol/(mg·pr)、(283.6±25.3)ng/L及(518.5±42.2)ng/L,细胞活力及SOD含量分别显著增加为(89.00±7.50)%及(19.1±2.2)U/(mg·pr)。结论虎杖苷显著抑制LPS诱导的成纤维细胞凋亡、氧化应激及炎性反应,并促进细胞增殖。
Objective To investigate the protective effect of polydatin on the apoptosis,oxidative stress,and inflammatory response of skin fibroblasts induced by lipopolysaccharide(LPS).Methods From January to April,2021,the fibroblasts were stimulated by 5 mg/L LPS to establish the experimental models,and were randomly divided into 4 groups:the cells in the normal control group received no treatment;the cells in the drug control group were treated with 50μmol/L polydatin;the cells in the experimental group were treated with 5 mg/L LPS;and the cells in the treatment group were treated with 5mg/L LPS and 50μmol/L polygonin.The cell proliferation and cytotoxicity assay kit(CCK-8)was used to detect the cell viability.The cell apoptosis was detected by terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling(TUNEL)assay.The malonaldehyde(MDA)and superoxide dismutase(SOD)contents in the cells were detected by the commercial kits.The enzyme-linked immunosorbent assay(ELISA)kits were used to detect the levels of tumor necrosis factorα(TNFα)and interleukin-6(IL-6)in the culture medium.Results Compared with those in the normal control group,the apoptosis rate and the contents of MDA,TNFα,and IL-6 in the culture medium were respectively increased to(12.7±0.11)%,(3.38±0.29)nmol/(mg·pr),(483.5±38.4)ng/L,and(784.4±49.6)ng/L and the cell viability and SOD content were respectively decreased to(78.00±5.90)%and(11.5±1.3)U/(mg·pr)in the experimental group.Compared with those in the experimental group,the cell apoptosis rate and the contents of MDA,TNFα,and IL-6 in the culture medium were respectively decreased to(7.5±0.08)%,(2.18±0.19)nmol/(mg·pr),(283.6±25.3)ng/L,and(518.5±42.2)ng/L and the cell viability and SOD content were respectively increased by(89.00±7.5)%and(19.1±2.2)U/(mg·pr)in the treatment group.Conclusion Polygonin significantly inhibits the apoptosis,oxidative stress,and inflammatory response of fibroblasts induced by LPS,and promotes cell proliferation.
作者
陈白烨
林博
王学明
Chen Baiye;Lin Bo;Wang Xueming(Department of Plastic Surgery,Maternal and Child Health Care Hospital of Fujian Province,Fuzhou 350000,China;Department of Anesthesiology,First Affiliated Hospital of Fujian Medical University,Fuzhou 350000,China)
出处
《国际医药卫生导报》
2021年第21期3289-3292,共4页
International Medicine and Health Guidance News
基金
国家自然科学基金项目(82000459)。
