摘要
目的:通过网络药理学分析葶苈大枣泻肺汤中的主要成分对治疗慢性心力衰竭(Chronic Heart Failure,CHF)的作用机制。方法:利用中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)数据库筛选药物的主要成分及其相关靶点;在GeneCards数据库搜索与CHF有联系的疾病基因;在此基础上通过STRING平台进行蛋白质相互作用(Protein-Protein Interaction,PPI)分析并构建PPI网络;通过Cytoscape构建葶苈大枣泻肺汤对CHF的"药物成分—靶点—疾病"网络;最后采用Metascape数据库及R语言对其进行基因本体论(The Gene Ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析。结果:筛选出葶苈大枣泻肺汤中25种主要成分及187个相关靶点,CHF的疾病靶点618个,通过韦恩图获得共同靶点,并在STRING平台上进行蛋白质网络分析,显示葶苈大枣泻肺汤治疗CHF可能与多种靶点、生物过程及通路相关,对CHF的靶点显著富集于晚期糖基化终末产物-晚期糖基化终末产物受体(Advanced Glycation End Products-RAGE,AGE-RAGE)信号通路、流体剪切应力和动脉粥样硬化信号通路、肿瘤坏死因子(Tumor Necrosis Factor,TNF)信号通路、癌症信号通路等相关。结论:葶苈大枣泻肺汤对治疗CHF具有多靶点、多途径的作用机制,为临床治疗提供参考。
Objective:To analyze the mechanism of the main components of the Tingli Dazao Xiefei decoction(葶苈大枣泻肺汤on CHF based on network pharmacology.Methods:The main components of the medicines and their related targets were screened in TCMSP database,the disease genes of related to CHF were searched in GeneCards database,protein-protein interaction was analyzed and PPI network was conducted through the STRING platform.The pharmaceutical component-target-disease network of the Tingli Dazao Xiefei decoction on CHF was constructed by Cytoscape software.Finally,GO analysis and KEGG enrichment analysis were executed through Metascape database and R language.Results:25 main components and 187 related targets in the Tingli Dazao Xiefei decoction were screened,with 618 disease targets of CHF.Common targets were obtained through Venn diagram,the results of PPI network analysis showed that the Tingli Dazao Xiefei decoction on CHF may be associated with multiple targets,biological processes and pathways,and the targets of the Tingli Dazao Xiefei decoction on CHF were significantly enriched in AGE-RAGE signaling pathway,fluid shear stress and atherosclerosis signaling pathway,TNF signaling pathway,and cancer signaling pathway,etc..Conclusion:The Tingli Dazao Xiefei decoction shows a multi-target and multi-path mechanism on CHF,which provides a reference for clinical treatment of the disease.
出处
《中医临床研究》
2021年第20期1-5,共5页
Clinical Journal Of Chinese Medicine
关键词
网络药理学
葶苈大枣泻肺汤
慢性心力衰竭
靶点预测
Network pharmacology
The Tingli Dazao Xiefei decoction
Chronic heart failure
Targets prediction
