摘要
Dear Editor,For more than two decades,Arabidopsis thaliana plants have been routinely grown in vitro within transparent Petri dishes containing agar-based solid media.Although convenient to study root developmental,nutritional and morphological responses to abiotic cues and to elucidate gene functions following reverse and forward genetic approaches,this system exposes roots to light during illumination of the shoots.Recently,Zheng et al.(2019)raised serious concerns whether root growth behavior and molecular root reactions in this experimental set-up are solely due to the designedly and chemically defined growth substrate composition,in their case phosphate(Pi)deficiency,or due to light-triggered reactions that are artificially-induced by exposing roots to light.
基金
supported by Emmy Noether grant 1668/1-1 from the Deutsche Forschungsgemeinschaft to G.P.B.
