摘要
为摸清我国牛恶性卡他热(BMCF)的流行情况,用间接ELISA(i-ELISA)方法对采自新疆、青海和甘肃等27个省(市)养殖场、屠宰场和散养户的2731份牛血清进行了抗体检测。同时对选用试剂盒检测质控血清的性能、检测分析敏感性、检测方法的交叉反应等指标进行了验证。结果表明,共检测出阳性牛血清样本29份,阴性牛血清2702份,阳性率1.06%;按不同牛血清来源统计,牛恶性卡他热的规模场阳性率0.56%,散养户阳性率1.18%,屠宰场阳性率1.93%;按不同饲养方式统计,牛全舍饲饲养方式的牛恶性卡他热病毒抗体阳性率0.41%,半舍饲饲养方式的牛恶性卡他热病毒抗体阳性率0.35%,放牧饲养方式的牛恶性卡他热病毒抗体阳性率0.35%。说明我国养殖的牛群中存在牛恶性卡他热的感染,但感染流行率较低,个别省(市)呈散发状态。
To find out the epidemic situation of bovine malignant catarrhal fever(BMCF)in China,2731 bovine serum samples collected from farms,and free-range farmers in 27 provinces(cities)including Xinjiang,Qinghai,and Gansu were detected by indirect ELISA.At the same time,the performance,sensitivity,and cross-reaction of the kit were verified.The results showed that 29 bovine serum samples were positive and 2702 serum samples were negative,with a positive rate of 1.06%.According to the statistics of different sources of bovine serum,the positive rate was 0.56%in large-scale farms,1.18%in free-range households,and 1.93%in slaughterhouses.According to different feeding methods,the positive rate of bovine malignant catarrhal fever antibody was 0.41%in full house feeding,0.35%in half house feeding,and 0.35%in grazing feeding.In conclusion,there was infection of bovine malignant catarrhal fever in cattle farms in China,but the prevalence rate was low and sporadic in some provinces and cities.
作者
孙雨
王睿男
李晓霞
孙航
魏巍
刘颖昳
冯冰
陈玲
顾小雪
王传彬
SUN Yu;WANG Ruinan;LI Xiaoxia;SUN Hang;WEI Wei;LIU Yingyi;FENG Bing;CHEN Ling;GU Xiaoxue;WANG Chuanbin(China Animal Disease Control Center,Beijing 102600,China)
出处
《中国草食动物科学》
CAS
2021年第6期24-28,共5页
China Herbivore Science
基金
十三五国家重点研发计划(2016YFD0500900)
现代农业人才支撑计划(2130106-024)
跨境动物疫病防控机制建设(2130114-FK)。
关键词
牛恶性卡他热
抗体检测
血清学调查
bovine malignant catarrhal fever
antibody detection
serological investigation
