摘要
目的探讨应用酶联抗体检测系统性红斑狼疮(SLE)患者血清抗双链DNA(dsDNA)抗体、抗核小体抗体(AnuA)、抗史密斯(Sm)抗体及抗磷脂抗体的效能及评估患者疾病活动度的价值。方法选取该院收治的88例SLE患者(SLE组)及88例体检健康者(对照组),应用酶联抗体检测两组血清抗dsDNA抗体、AnuA、抗Sm抗体、抗β2糖蛋白1(β2GP1)抗体、抗心磷脂抗体(ACA)水平,比较SLE疾病不同活动度患者各血清抗体水平,采用Pearson相关分析各血清抗体与系统性红斑狼疮疾病活动性指数(SLEDAI)的相关性,绘制受试者工作特征(ROC)曲线分析各血清抗体诊断SLE及不同疾病活动度的效能。结果SLE组抗dsDNA抗体、AnuA、抗Sm抗体、IgG-β2GP1、IgM-β2GP1、IgG-ACA、IgM-ACA水平均高于对照组(P<0.05);ROC曲线分析结果显示,抗Sm抗体诊断SLE的曲线下面积(AUC)最大,为0.839;AnuA评估SLE轻、中度活动的AUC最大;抗dsDNA抗体评估SLE重度活动的AUC最大;相关性分析显示,抗dsDNA抗体、AnuA、IgG-β2GP1、IgM-β2GP1、IgG-ACA、IgM-ACA均与SLEDAI评分呈正相关(P<0.05)。结论SLE患者血清抗dsDNA抗体、AnuA、抗Sm抗体及抗磷脂抗体水平明显升高,且血清抗dsDNA抗体、AnuA及抗磷脂抗体与SLEDAI评分呈正相关,应用酶联抗体检测SLE患者各项血清抗体对SLE的诊断、疾病活动度评估具有重要意义。
Objective To investigate the efficacy of using enzyme-linked antibodies to detect serum anti-double-stranded DNA(dsDNA)antibody,anti-nucleosome antibody(AnuA),anti-Smith(Sm)antibody and anti-phospholipid antibody in patients with systemic lupus erythematosus(SLE)and the value of evaluating the patient′s disease activity.Methods A total of 88 SLE patients(SLE group)and 88 healthy subjects(control group)were selected from the hospital.Enzyme-linked antibody were used to detect serum anti-dsDNA antibody,AnuA,anti-Sm antibody,anti-β2 glycoprotein 1(β2GP1)antibody,and anti-cardiolipin antibody(ACA)in the two groups.The serum antibody levels of patients with different activities of SLE were compared,the correlation between each serum antibody and SLEDAI score were analyzed by Pearson correlation,and the efficiency of diagnosing SLE and different disease activities were analyzed by drawing ROC curve.Results The levels of anti-dsDNA antibody,AnuA,anti-Sm antibody,IgG-β2GP1,IgM-β2GP1,IGg-ACA and IgM-ACA in SLE group were higher than those in control group(P<0.05).The area under curve(AUC)of anti-Sm antibody in SLE diagnosis was the highest at 0.839,the AUC of AnuA in the evaluation of mild and moderate activity of SLE patients was the largest,and the AUC of severe activity of SLE patients evaluated by anti-dsDNA antibody was the largest.Anti-dsDNA antibody,AnuA,IgG-β2GP1,IgM-β2GP1,IgG-ACA,IgM-ACA were positively correlated with SLEDAI score(P<0.05).Conclusion The serum levels of anti-dsDNA antibody,AnuA,anti-Sm antibody,and anti-phospholipid antibody in SLE patients were significantly increased,and the levels of anti-dsDNA antibody,AnuA and anti-phospholipid antibody were positively correlated with SLEDAI score,which are of great value for the diagnosis of SLE and the evaluation of disease activity.
作者
赖立川
吴荣才
蒙丹丽
王浜琴
LAI Lichuan;WU Rongcai;MENG Danli;WANG Bangqin(Department of Clinical Laboratory,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530021,China;Department of Rheumatology,the People′s Hospital of Guangxi Zhuang Autonomous Region,Nanning,Guangxi 530021,China)
出处
《国际检验医学杂志》
CAS
2021年第22期2735-2739,2744,共6页
International Journal of Laboratory Medicine
基金
广西壮族自治区卫生厅自筹经费科研课题(Z20201240)。
关键词
系统性红斑狼疮
抗双链DNA抗体
抗磷脂抗体
疾病活动度
systemic lupus erythematosus
anti-double-stranded DNA
anti-phospholipid antibody
disease activity
