摘要
【目的】明确杉树林下三七根系生长不良的原因,为林下三七种植过程中土壤的选择提供理论依据。【方法】以杉树林下三七生长正常区域的土壤为对照,测定生长不正常区域的土壤的电导率、pH和酚类化感物质及三七根系生长指标(须根数量、主根长度、鲜重和干重);采用滤纸片法,研究不同浓度酚类物质(1、5、10、15、20和30 mg/L)对三七根系活力和相对电导率的影响,以及对生菜根系生长的抑制作用。【结果】三七生长不正常区域的土壤显著降低三七的须根数量、主根长度和根系的鲜重与干重(P<0.05,下同)。三七生长不正常区域的土壤pH显著降低,土壤电导率显著升高,对羟基苯甲酸、香草醛、丁香酸、阿魏酸和香草酸含量升高,且能显著抑制化感指示植物生菜根系的生长。相关分析结果显示,三七的须根数量、鲜重和干重均与土壤pH呈显著或极显著(P<0.01,下同)正相关,与土壤电导率呈显著或极显著负相关;土壤中对羟基苯甲酸和香草酸含量与三七的鲜重和干重呈显著负相关。相比CK,当丁香酸、阿魏酸、香草酸及混配的浓度介于5~30 mg/L时,根系活力显著降低,当香草醛、丁香酸和阿魏酸的浓度达15 mg/L时,根系溶液的相对电导率显著升高。【结论】杉树林下土壤pH的降低和酚类化感物质的累积会对三七生长产生不利影响。
【Objective】To reveal the reasons of unhealthy growth of Panax notoginseng root under the forest of Cunninghamia lanceolata(Lamb.)Hook,and provide a theoretical basis for soil selection in the process of planting P.notoginseng.【Method】The soil electrical conductivity(EC),p H and phenols allelopathic substances and the growth index of P.notoginseng root(number of fibrous roots,length of main roots,fresh weight and dry weight)in unhealthy growth area were measured,and healthygrowth area treatment control.The effects of different concentrations of phenols(1,5,10,15,20,30 mg/L)on root activity and relative conductivity of tissue extracts of P.notoginseng were studied by using filter The number of fibrous roots,length of main roots,the fresh and dry weight of root system of P.notoginseng decreased significantly in unhealthy growth soil(P<0.05,the same below).The pH value of the soil in the unhealthy growth area of P.notoginseng was significantly decreased,but the EC value was significantly increased,and the content of phenols(phydroxybenzoic acid,vanillin,syringic acid,ferulic acid and vanillic acid)wasincreased.The root growth of lettuce in unhealthy soil was significantly inhibited.Pearson correlation analysis showed that the number of fibrous roots,fresh weight and dry weight of P.notoginseng were significantly or extremely(P<0.01,the same below)positively correlated with soil pH but significantly or extremely negatively correlated with EC values.The contents of p-hydroxybenzoic acid and vanillic acid in soil were significantly negatively correlated with fresh weight and dry weight of P.notoginseng.Compared with CK,when the concentrations of syringic acid,ferulic acid,vanillic acid and the mixture were between 5 and30 mg/L,the root activity decreased significantly.When the concentrations of vanillin,syringic acid and ferulic acid reached15 mg/L,the relative conductivity of root solution increased significantly.【Conclusion】The decrease of soil pH and accumulation of phenolic allelochemicals under the forest of C.lanceolata have the adverse effects on the growth of P.notoginseng.
作者
张义杰
陆仁窗
李天尧
叶辰
范黎明
杨敏
黄惠川
刘屹湘
何霞红
朱书生
ZHANG Yi-jie;LU Ren-chuang;LI Tian-yao;YE Chen;FAN Li-ming;YANG Min;HUANG Hui-chuan;LIU Yi-xiang;HE Xia-hong;ZHU Shu-sheng(College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China;Yunnan University,Kunming 650091,China;Southwest Forestry University,Kunming 650224,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2021年第8期2096-2105,共10页
Journal of Southern Agriculture
基金
国家重点研发计划项目(2017YFC1702502)
云南绿色食品国际合作研究中心项目(2019ZG00901-02)
云南省中国工程院云南院士工作站项目(2018IC063)。
关键词
杉树
三七
酚类物质
化感作用
Cunninghamia lanceolata(Lamb.)Hook
Panax notoginseng
phenols
allelopathy
