摘要
SVP(short vegetative phase)是一类开花抑制基因,通过调节开花相关基因的表达,影响植物从营养生长阶段向生殖生长阶段的转变进程。根据公布的菠萝基因组信息,从‘台农4号’菠萝中克隆到2个AcSVP基因AcSVP1和AcSVP2。结果表明:AcSVP1和AcSVP2分别编码225和230个氨基酸,均含有MADS-box、K-box保守结构域,二者所编码蛋白均属MADS-box基因家族成员。定量PCR分析结果显示,2个AcSVP基因在菠萝茎尖、茎基和叶中均有不同程度的表达,乙烯利处理后主要表现为下调。乙烯利处理8 h内,AcSVP1在茎尖、叶中的表达显著下调,但在茎基组织中呈现先下调后上升的趋势;乙烯利处理后8 h,茎基组织中AcSVP1的相对表达量略高于对照。与AcSVP1不同,乙烯利处理8 h内AcSVP2在茎尖、茎基、叶3种组织中均明显下调;乙烯利处理后8 h,AcSVP2在茎尖、茎基、叶组织中的相对表达量只有对照的8%、44%和33%。SVP是目前已知的最重要的一类开花抑制基因,AcSVP在响应乙烯利的过程中表达显著下调,表明其在乙烯利诱导菠萝成花过程中可能发挥重要作用。
SVP(short vegetative phase)is a type of flowering suppressor gene,which regulates the expression of flowering-related genes to affect the transition process of plants from the vegetative growth stage to the reproductive growth stage.According to the published pineapple genome information,two AcSVP genes named AcSVP1 and AcSVP2 were cloned in‘Tainong 4’pineapple.Analyses showed that AcSVP1 and AcSVP2 encoded 225 and 230 amino acids,respectively,and both contained MADS-box and K-box conserved domains.The proteins encoded belonged to the MADS-box gene family members.qRT-PCR analysis showed that the genes were expressed in different degrees in stem tip,stem base and leaf tissues,and after ethephon treatment the genes were mainly down-regulated to varying degrees compared with the control.Within 8 h of ethephon treatment,AcSVP1 was significantly down-regulated in the stem tips and leaves while in the stem-basal tissue which showed a trend of first down and then up-regulation,and the relative expression of AcSVP1 in the stem-basal tissues was slightly higher than that of its control after 8 h of ethephon treatment.Different from AcSVP1,AcSVP2 gene was significantly down-regulated in stem tip,stem base and leaf tissues within 8 h of ethephon treatment,and the relative expression level of AcSVP2 in the stem tip,stem base and leaf tissues was respectively about 8%,44%and 33%of the control after 8 h of ethylene treatment.SVP was the most important type of flowering suppressor gene.And the down-regulated of AcSVP in response to ethephon stimulation indicated that AcSVP maybe play a key role in the flowering processes of pineapple in responses to ethephon signal.
作者
李玉静
陈哲
胡福初
阮城城
罗志文
王祥和
范鸿雁
张治礼
LI Yujing;CHEN Zhe;HU Fuchu;RUAN Chengcheng;LUO Zhiwen;WANG Xianghe;FAN Hongyan;ZHANG Zhili(Institute of Life Sciences and Pharmacy,Hainan University,Haikou,Hainan 570228,China;Institute of Tropical Fruit Trees,Hainan Academy of Agricultural Sciences/Key Laboratory of Tropical Fruit Tree Biology of Hainan Province/Haikou Investiga-tion Station of Tropical Fruit Trees,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571100,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第10期2806-2812,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31960589)
海南省科协青年科技英才学术创新计划项目(No.QCXM201803)。
