摘要
选择12个代表性的乌龙茶品种(种质)作为供试材料,基于SPME-GC-MS方法获得香气物质表型数据,探索乌龙茶核心种质构建技术流程。当取样比例为25%时,采用多次聚类随机取样法,可以获得‘金牡丹’、‘铁观音’、‘悦茗香’3个核心种质,乙酸苄酯、香叶醇、橙花叔醇、吲哚、芳樟醇及其氧化物等7个核心香气物质。结合SSR标记基因型数据,进行遗传多样性差异分析,核心种质与原生种质2个种群间,在7个表型性状上无显著遗传差异。初步搭建成乌龙茶核心种质构建与遗传评估分析技术流程。
Twelve representative oolong tea cultivars(Camellia sinensis)were used to establish a process for constructing a germplasms classification based on the phenotypic SPME-GC-MS database of the aromatic substances in the plants.At a sampling rate of 25%,Tieguanyin,Yuemingxiang,and Jinmudan were identified as the core germplasms,while linalool oxide I,linalool,caproic acid(Z)-3-hexene ester,aromatic alcohol oxideⅢbenzyl acetate,geraniol,nerolidol,and indolecore the principal aroma metabolites.In addition to the existing SSR marker genotype data,the genetic diversity analysis showed no significant differentiations on 7 phenotypic traits between the core and the primary germplasms.A prototype process based on SSR markers and aromatics in oolong tea plants for constructing a systematic germplasms classification was established.
作者
孔祥瑞
杨军
林梓溪
马定国
王让剑
陈常颂
KONG Xiang-rui;YANG Jun;LIN Zi-xi;MA Ding-guo;WANG Rang-jian;CHEN Chang-song(Tea Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;National Tea Quality and Safety Engineering and Technology Research Center,Anxi,Fujian 362441,China;Fu’an Blue Sea and Blue Sky Agricultural Technology Development Co.,Ltd.,Fu’an,Fujian 355000,China)
出处
《茶叶学报》
2021年第3期105-111,共7页
Acta Tea Sinica
基金
“5511”协同创新工程(XTCxGc2021004)
福建省现代农业(茶叶)产业技术体系专项(闽财指[2020]786号)
福建省科技厅星火计划项目(2019S0051)。
关键词
乌龙茶
核心种质
分析流程
香气物质
遗传多样性
Oolong tea
core germplasm
analysis process
aroma metabolites
genetic diversity
