摘要
为选择适合准确鉴定铁皮石斛的DNA条形码,以25份铁皮石斛样品和10份其他不同种类石斛为实验材料,利用目前常用的9条植物DNA条形码(ITS2、nad1、matK、ycbL、psbK-psbI、trnL-trnF、trnG-trnS、rbcL和trnH-psbA)对这些石斛材料进行分子鉴定。结果显示,9条DNA条形码的扩增率和测序成功率均很高,通用性很强。其中,rbcL与trnH-psbA的种内最大遗传距离大于种间最小遗传距离,不符合条形码的基本要求,其余条形码遗传距离均符合种内最大遗传距离小于种间最小遗传距离的要求。种间变异顺序为ITS2>trnL-trnF>trnG-trnS>psbK-psbI>ycf1b>matK>nad1,其中ITS2的进化速率最快,变异率最高(25.1%)。ITS2和trnL-trnF对本实验中石斛材料的分辨率达到90.1%,建议ITS2+trnL-trnF作为铁皮石斛种质资源鉴定的DNA条形码。本研究为利用DNA条形码技术鉴别铁皮石斛及其近源种石斛提供了参考和分子依据。
In order to select a suitable DNA barcode for accurate identification of Dendrobium officinale,25 samples of D.officinale and 10 samples of other Dendrobium species were subjected to molecular identification using nine commonly used plant DNA barcodes(ITS2,nad1,matK,ycbL,psbK-psbI,trnL-trnF,trnG-trnS,rbcL and trnH-psbA).The results showed that the versatility of the nine DNA barcodes was strong,with high success rates of amplification and sequencing.The maximum genetic distance within species of rbcL and trnH-psbA was greater than the minimum genetic distance between species,which did not meet the basic requirements for barcodes.Instead,the maximum genetic distance within species of the other barcodes was smaller than the minimum genetic distance between species.The decreasing order of interspecific variation was ITS2>trnL-trnF>trnG-trnS>psbK-psbI>ycf1b>matK>nad1.ITS2 had the fastest evolution rate and the highest mutation rate(25.1%).The identification rates of ITS2 and trnL-trnF for the Dendrobium samples tested were both 90.1%.It is recommended that ITS2+trnL-trnF be used for the identification of D.officinale germplasm resources.The results of this study are expected to provide a reference and molecular basis for the identification of D.officinale and its closely related species by using DNA barcoding technology.
作者
陈文强
汪小福
陈笑芸
彭城
徐俊锋
蔡健
CHEN Wenqiang;WANG Xiaofu;CHEN Xiaoyun;PENG Cheng;XU Junfeng;CAI Jian(State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Biological and Food Engineering,Fuyang Normal University,Fuyang 236037,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2021年第22期131-139,共9页
Food Science
基金
国家自然科学基金面上项目(31772098)
安徽省高校自然科学研究重点项目(KJ2018A0352)。
关键词
铁皮石斛
DNA条形码
分子鉴定
遗传距离
Dendrobium officinale
DNA barcoding
molecular identification
genetic distance
