摘要
目的探讨回阳生肌膏对微血管内皮细胞的影响及其促进创面修复的作用机制。方法体外实验:将细胞分为4组,正常组:正常内皮细胞,模型组:病变内皮细胞,实验组:病变内皮细胞+回阳生肌膏,对照组:病变内皮细胞+龙珠软膏,检测肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、内皮型一氧化氮合酶(eNOS)的表达量。体内实验:将大鼠分为3组,正常组:正常大鼠,模型组:糖尿病大鼠+凡士林纱条,实验组:糖尿病大鼠+回阳生肌膏纱条。观察创面愈合及形态变化,检测血清中TNF-α、血管内皮生长因子(VEGF)的表达量。结果体外实验:与正常组比较,模型组eNOS的表达量较高,差异有统计学意义(P>0.05),与模型组比较,实验组和对照组中eNOS的表达量较低,差异无统计学意义(P>0.05),与对照组比较,实验组微血管中eNOS表达较高,差异无统计学意义(P>0.05)。与正常组比较,模型组中TNF-α的表达量较高,差异有统计学意义(P<0.05),与模型组比较,实验组中TNF-α的表达量较低,差异有统计学意义(P<0.05),与对照组比较,实验组中TNF-α的表达量较低,差异有统计学意义(P<0.05)。与正常组比较,模型组中IL-6的表达量较低,差异无统计学意义(P>0.05),与模型组比较,实验组中IL-6的表达量较高,差异有统计学意义(P<0.05),对照组中IL-6的表达量较高,差异无统计学意义(P>0.05),与对照组比较,实验组中IL-6的表达量较高,差异无统计学意义(P>0.05)。体内实验:第3天时,与正常组比较,模型组的创面愈合率较低,差异有统计学意义(P<0.05),与模型组比较,实验组创面愈合率较高,差异无统计学意义(P>0.05)。第7天时,与正常组比较,模型组的创面愈合率较低,差异有统计学意义(P<0.05),与模型组比较,实验组创面愈合率较高,差异有统计学意义(P<0.05)。第14天时,与正常组比较,模型组的创面愈合率较低,差异有统计学意义(P<0.05),与模型组比较,实验组创面愈合率较高,差异有统计学意义(P<0.05)。第3天时,与正常组比较,模型组中TNF-α的表达量较低,差异有统计学意义(P<0.05),与模型组比较,实验组中TNF-α的表达量较高,差异有统计学意义(P<0.05)。第7天时,与正常组比较,模型组中TNF-α的表达量较低,差异无统计学意义(P>0.05),与模型组比较,实验组中TNF-α的表达量较高,差异有统计学意义(P<0.05)。第14天时,与正常组比较,模型组中TNF-α的表达量较高,差异有统计学意义(P<0.05),与模型组比较,实验组中TNF-α的表达量较低,差异有统计学意义(P<0.05)。第3天、第7天时,与正常组比较,模型组中的VEGF表达量较低,差异有统计学意义(P<0.05),第14天时,与正常组比较,模型组中的VEGF表达量较低,差异有统计学意义(P<0.05)。第3天、第7天、第14天时,与模型组比较,实验组中的VEGF表达量较高,差异有统计学意义(P<0.05)。结论回阳生肌膏能通过增加eNOS、VEGF的表达和降低促炎因子的表达,维持内皮细胞稳态,从而降低炎症反应,促进血管再生和创面修复,且效果优于龙珠软膏。
Objective To explore the mechanism of Huiyang Shengji Ointment on inflammatory reaction of microvascular endothelial cells and improving repair of wound.Methods In vitro experiment: the cells were divided into four groups: normal group(normal endothelial cell),model group(diseased endothelial cell),experimental group(diseased endothelial cell + Huiyang Shengji ointment),and control group(diseased endothelial cell + Longzhu Ointment).The expression of tumor necrosis factor-α(TNF--α),interleukin-6(IL6) and endothelial nitric oxide synthase(eNOS) were detected.In vivo experiment: the rats were divided into three groups: normal group;model group(diabetic rats + Vaseline gauze) and experimental group(diabetic rats + Huiyang Shengji Ointment gauze).The healing and morphological changes of the sore surface were observed, and the serum of rats was taken to detect the expression of TNF-α and vascular endothelial growth factor(VEGF).Results In vitro experiment: compared with the normal group, the expression of eNOS in the model group was increased significantly and the difference was not statistically significant(P>0.05);compared with the model group, the expression of eNOS in the experimental group and control group was higher and the difference was not statistically significant(P>0.05);and compared with the control group, the expression of eNOS in the experiment group was increased significantly and the difference was not statistically significant(P>0.05).Compared with the normal group, the expression of TNF-αin the model group was increased significantly and the difference was statistically significant(P<0.05);compared with the model group, the expression of TNF-αin the experimental group was lower and the difference was statistically significant(P<0.05);compared with the control group, the expression of TNF-αin the experimental group was lower and the difference was statistically significant(P<0.05).Compared with the normal group, the expression of IL-6 in the model group were significantly lower and the difference was not statistically significant(P>0.05);compared with the model group, the expression of IL-6 in the experimental group were significantly higher and the difference was statistically significant(P>0.05);compared with the control group, the expression of IL-6 in the experimental group were significantly higher than that in the normal group and the difference was not statistically significant(P>0.05).In vivo experiment: on the 3 rd, compared with the normal group, the wound healing rate in the model group was significantly higher and there was significant statistical difference(P< 0.05);and compared with the model group, the wound healing rate in the experimental group was significantly higher and there was no significant statistical difference(P> 0.05).on the 7 th day, compared with the normal group, the wound healing rate in the model group was significantly lower and there was significant difference(P<0.05), compared with the model group, the wound healing rate in the experimental group was significantly higher and the difference was statistically significant(P<0.05).On the 14 th day, compared with the normal group, the wound healing rate in the experimental group was significantly lower in the model group and the difference was statistically significant(P<0.05);compared with the model group, the wound healing rate in the experimental group was significantly higher and the difference was statistically significant(P< 0.05).On the 3 rd day, Compared with the normal group, the expression of TNF-α in the model group was significantly lower than and the difference was statistically significant(P<0.05);compared with the model group, the expression of TNF-α in the experimental group was significantly higher, and the difference was statistically significant(P<0.05).On the 7 th day, Compared with the normal group, the expression of TNF-α in the model group was significantly lower than and the difference was statistically significant(P<05);compared with the model group, the expression of TNF-α in the experimental group was significantly higher, and the difference was statistically significant(P<0.05).On the 14 th day, compared with the normal group, the expression of TNF-α in the model group was significantly higher and the difference was statistically significant(P<0.05);compared with the model group, the expression of TNF-α in the experimental group was significantly lower, and the difference was statistically significant(P<0.05).On the 3 rd day and 7 th day, compared with the normal group, the expression of VEGF in the model group was significantly lower and the difference was statistically significant(P<0.05).On the 14 th day, compared with the normal group at the same time, the expression of VEGF in the experimental group was significantly lower and the difference was statistically significant(P<0.05).On the 3 rd, 7 th, and 14 th day, compared with the model group, the difference was statistically significant(P<0.05).Conclusion Huiyang Shengji Ointment can maintain endothelial cell homeostasis by increasing the expression of eNOS and VEGF and reducing the expression of pro-inflammatory factors, thus reducing inflammatory reaction and promoting vascular regeneration and wound repair with better effect than Longzhu Ointment.
作者
吴黎
郭卉
杨易森
徐旭英
WU Li;GUO Hui;YANG Yi-sen;XU Xu-ying(Beijing University of Chinese Medicine,Beijing 100025,China;Capital Medical University,Beijing 100069;Surgical Department of Pyocutaneous Vascular Diseases,Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical University,Beijing 100005)
出处
《北京中医药》
2021年第9期956-962,共7页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81673975)。
