摘要
采用8种低毒溶剂并结合超声波提取铃兰中总强心苷,通过单因素实验优选出提取工艺中溶剂种类、料液比、超声提取时间、提取温度4个因素的最佳条件,并通过正交实验探究最佳提取条件。最终优化得到以80%乙醇作为提取溶剂,料液比1∶10、超声提取时间120min、提取温度70℃时总强心苷得率最高,为0.25%。
Eight kinds of low toxic solvents combined with ultrasonic wave were used to extract total cardiac glycosides from Convallaria keiskei Miq..The optimum extraction conditions of solvent type,solid-liquid ratio,ultrasonic extraction time and extraction temperature were optimized by single factor experiment,and the optimum extraction conditions were explored by orthogonal experiment.Finally,80%ethanol was used as extraction solvent,the optimal parameters of ultrasonic assisted were liquid to material ratio of 1∶10(1g∶10mL),ultrasonic time of 120min,ultrasonic temperature of 70℃,the extracting rate of total cardiac glycosides was 0.25%.
作者
黄泰奇
李成成
朱锐
黄启东
金卫华
HUANG Tai-qi;LI Cheng-cheng;ZHU Rui;HUANG Qi-dong;JIN Wei-hua(College of Life Science&Chemistry,Wuhan Donghu University,Wuhan 430212,China)
出处
《化学工程师》
CAS
2021年第11期78-81,51,共5页
Chemical Engineer
基金
武汉东湖学院“东湖未来之星”大学生科研项目(2020)。
关键词
铃兰
总强心苷
超声波提取
Convallaria keiskei Miq.
total cardiac glycosides
ultrasonic extraction
