摘要
【目的】比较持续母乳喂养条件下不同分娩方式的34周龄婴儿肠道菌群差异,探讨分娩方式对较大婴儿肠道菌群发育的影响。【方法】在北京地区招募健康足月分娩母乳喂养婴儿,在34周仍然参与随访的持续母乳喂养婴儿共21例,其中剖宫产婴儿16例、阴道分娩婴儿5例,进行肠道菌群的16S rRNA检测。【结果】两组共21个粪便样本中,共注释到6个门,分别为:疣微菌门、变形菌门、梭杆菌门、厚壁菌门、放线菌门和拟杆菌门;两组共21个样本中共有57个OTU注释到属水平,其中,26个属水平OTU被注释到厚壁菌门,18个属水平OTU被注释到变形菌门,6个属水平OTU被注释到放线菌门,5个属水平OTU被注释到拟杆菌门,梭杆菌门、疣微菌门各有1个属水平OTU被注释。其中变形菌门在阴道分娩组(44.17%)肠道菌群中的含量高于剖宫产组(16.10%);而放线菌门在阴道分娩婴儿(0.00%)肠道菌群中的含量低于剖宫产婴儿(0.09%)。阴道分娩组与剖宫产组相比,共有7个菌属的丰度发生了显著降低(P<0.05),分别为副杆菌属、葡萄球菌属、嗜血杆菌属、乳杆菌属、肠球菌属、双歧杆菌属及一注释到科水平的毛螺旋菌科OTU。【结论】分娩方式对持续母乳喂养的婴儿肠道菌群结构存在影响,且这种影响在出生后34周仍然存在。
[Objective]To compare the differences in gut microbiota in 34-week-old infants under continuous breastfeeding with different delivery modes,and to explore the impact of delivery modes on the development of infant gut microbiota.[Methods]Healthy full-term breast-fed infants were recruited and 21 infants were still participating in follow-up at 34 weeks,including 16 infants delivered by cesarean and 5 vaginal delivered infants.The 16S rRNA of the gut microbiota was detected.[Results]The two groups of 21 samples contained 6 phyla:Actinobacteria,Bacteroides,Firmicutes,Fusobacteria,Proteobacteria and Verrucomicrobia;57 genera were found.Among them,there are 26 genera in Firmicutes,18 genera in Proteobacteria,6 genera in Actinomycota,5 genera in Bacteroides,1 genera in Fusobacteria and 1 genera Verrucomicrobia.Among them,the content of Proteobacteria in the gut microbiota of the vaginal delivery group(44.17%)was higher than that in the cesarean section(16.10%);and the content of Actinomycetes in the gut microbiota of vaginal delivery infants(0.00%)was lower than cesarean delivery babies(0.09%).At the genus level,compared with the cesarean section group,the abundance of a total of 7 microorganisms in the vaginal delivery group was significantly reduced(P<0.05),namely Staphylococcus,Lactobacillus,Parabacteroides,Enterococcus,Haemophilus,Bifidobacterium and an OTU of Enterobacteriaceae.[Conclusion]The mode of delivery has an impact on gut microbiota of infants continued breastfeeding,and this impact still exists at 34 weeks after birth.
作者
方圆
李玭
武微
熊倩
律娜
朱宝利
张玉梅
Yuan Fang;Pin Li;Wei Wu;Qian Xiong;Na Lü;Baoli Zhu;Yumei Zhang(CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Public Health,Peking University,Beijing 100191,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2021年第11期3642-3652,共11页
Acta Microbiologica Sinica
基金
国家重点研发计划(2018YFC1603803,2017YFD0400602)。
关键词
肠道菌群
婴儿
分娩方式
gut microbiota
infant
delivery mode
