摘要
目的分析人呼吸道合胞病毒(human respiratory syncytial virus,HRSV)感染HEp-2细胞后差异性表达的基因,为发现HRSV防治的潜在靶点提供线索。方法在公共基因表达数据库(gene expression omnibus,GEO)中收集HRSV感染的HEp-2细胞表达谱数据。运用R语言分析在HRSV感染4、8、12和15 h均表现出差异表达的基因,对其进行GO分析、KEGG通路分析、蛋白-蛋白相互作用(protein-protein interaction,PPI)网络构建等。以HRSV感染HEp-2细胞4 h后,随机选择PPI中参与蛋白互作相对较高的基因在转录水平进行qRT-PCR验证。结果筛选出101个差异表达基因,其中表达上调的有92个,表达下调的有9个。功能富集分析发现HRSV感染引起HEp-2细胞中免疫应答及其信号转导等多条信号通路产生明显变化。选择的6个差异表达基因qRT-PCR验证结果与转录组数据趋势一致。结论HRSV感染HEp-2细胞引起的基因差异表达及信号通路变化,对HRSV致病分子机制及防治策略研究具有一定意义。
Objective To find clues potentially valuable for fighting against infection with human respiratory syncytial virus(HRSV),the differentially expressed genes in HEp-2 cells infected with HRSV were analyzed.Methods Gene expression profiles of HEp-2 cells infected with HRSV were collected from the public gene expression omnibus(GEO)database.The differentially expressed genes following HRSV infection at each time point of 4,8,12,and 15 hours were found using R language.The differentially expressed genes were analyzed by gene ontology(GO),KEGG pathway and protein-protein interaction network(PPI).Genes with relatively high protein interaction in PPI were randomly selected for quantitative reverse transcription-polymerase chain reaction(qRT-PCR)verification at the transcription level from HEp-2 cells after HRSV infection at 4 hours.Results A total of 101 differentially expressed genes were determined,including 92 upregulated genes and 9 downregulated genes.Function enrichment analysis revealed that HRSV infection could cause significant changes in multiple signaling pathways such as immune response in HEp-2 cells.The results of qRT-PCR were consistent with the trend of transcriptome data.Conclusions The differentially expressed genes and the change of signaling pathways in HRSV-infected HEp-2 cells is of great significance to the studies on pathogenic mechanism and prevention of HRSV infection.
作者
苏彦斌
刘勇
王烨
王朔
薛君宇
张天笑
彭向雷
郑妍鹏
张丽姝
付远辉
何金生
Su Yanbin;Liu Yong;Wang Ye;Wang Shuo;Xue Junyu;Zhang Tianxiao;Peng Xianglei;Zheng Yanpeng;Zhang Lishu;Fu Yuanhui;He Jinsheng(College of Life Sciences&Bioengineering,Beijing Jiaotong University,Beijing 100044,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2021年第5期575-580,共6页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金(81771777)。
关键词
人呼吸道合胞病毒
生物信息学
信号通路
Human respiratory syncytial virus
Bioinformatics
Signaling pathway
