摘要
目的探讨circPTPRA对乳腺癌细胞增殖、迁移和侵袭的影响及可能机制。方法收集41例乳腺癌患者的癌组织和对应的癌旁组织,RT-qPCR检测组织中circPTPRA和miR-556-3p表达水平。体外培养乳腺癌细胞MDA-MB-231,分别转染pcDNA-circPTPRA或anti-miR-556-3p或共转染pcDNA-circPTPRA与miR-556-3p后,CCK-8法检测细胞增殖,Transwell法检测细胞迁移和侵袭。双荧光素酶报告基因实验验证circPTPRA和miR-556-3p靶向关系。结果乳腺癌组织中circPTPRA表达低于癌旁组织(P<0.05),而miR-556-3p表达高于癌旁组织(P<0.05)。过表达circPTPRA或敲减miR-556-3p后,MDA-MB-231细胞OD值、迁移数和侵袭数降低(P<0.05)。circPTPRA靶向负调控调控miR-556-3p表达。过表达miR-556-3p逆转过表达circPTPRA对MDA-MB-231细胞增殖、迁移和侵袭的抑制作用。结论过表达circPTPRA可能靶向下调miR-556-3p阻碍乳腺癌细胞MDA-MB-231增殖、迁移和侵袭。
Objective To investigate the effect of circPTPRA on the proliferation,migration and invasion of breast cancer cell and its possible mechanism.Methods Cancer tissues and corresponding adjacent tissues of 41 breast cancer patients were collected,and the expression level of circPTPRA and miR-556-3p in the tissues were detected by RT-qPCR.Breast cancer cells MDA-MB-231 were cultured in vitro and transfected with pcDNAcircPTPRA,anti-miR-556-3p or co-transfected with pcDNA-circPTPRA and miR-556-3p.Cell proliferation was detected by CCK-8 method.Transwell was used to detect cell migration and invasion,and western blotting was used to detect the protein expression of Ki-67,MMP-2,and MMP-9 in the cells.The dual luciferase reporter gene experiment verified the targeting relationship between circPTPRA and miR-556-3p.Results The expression of circPTPRA in breast cancer tissues was lower than that of adjacent tissues(P<0.05),while the expression of miR-556-3p was higher(P<0.05).After overexpression of circPTPRA or knockdown of miR-556-3p,the cell OD value,the number of migration and invasion,and the protein expression of Ki-67,MMP-2 and MMP-9 in MDA-MB-231 cells were reduced(P<0.05).circPTPRA negatively regulated the expression of miR-556-3p.Overexpression of miR-556-3p reversed the inhibitory effect of overexpression of circPTPRA on the proliferation,migration and invasion of MDA-MB-231 cells.Conclusion Overexpression of circPTPRA may hinder the proliferation,migration and invasion of breast cancer cells MDA-MB-231 by down-regulating the expression of miR-556-3p.
作者
白文辉
李英
李宗龙
BAI Wenhui;LI Ying;LI Zonglong(Department of Breast Surgery,Qinghai Red Cross Hospital,Xining 810000,China)
出处
《实用医学杂志》
CAS
北大核心
2021年第20期2597-2601,共5页
The Journal of Practical Medicine
基金
青海省卫生健康委员会指导性课题(编号:2019-wjzdx-65)。
