摘要
随着对养殖水环境精准调控需求和生态环境保护意识的增强,养殖水体中氮、磷营养盐浓度的准确检测日趋重要。然而国内关于不同盐度养殖水体总氮、总磷标准检测方法的研究较为有限且未有定论。本文通过比较海洋监测规范:海水分析方法(过硫酸钾氧化法)、水质测定的标准方法(碱性过硫酸钾消解紫外分光光度法、钼酸铵分光光度法)以及联合消化法,探究盐度是否会对不同方法检测水体总氮、总磷浓度产生影响,从而筛选出适合不同盐度养殖水体的检测方法。结果表明,盐度对总氮测定有一定的干扰:过硫酸钾氧化法适合测定盐度大于18的水体,回收率在94.5%~108.0%;碱性过硫酸钾消解紫外分光光度法和联合消化法适合测定盐度小于4的水体,回收率在94.0%~108.9%。盐度对总磷测定没有明显干扰:但过硫酸钾氧化法测定盐度大于4的水体准确度更高,回收率在90.5%~105.5%;钼酸铵分光光度法和联合消化法测定盐度小于12的水体准确度更高,回收率在100.9%~107.0%。
With the increasing demand for precise regulation of aquacultural water environment and the enhancing awareness of ecological environmental protection,accurate detection of the concentrations of nitrogen and phosphorus nutrient in aquacultural water of different salinities is more and more important.However,domestic studies on the standard detection methods of total nitrogen and total phosphorus in water of different salinities are limited and inconclusive.In this study,the concentrations of total nitrogen and total phosphorus in water with different salinities were determined by seawater standard methods(potassium persulfate oxidation),water quality determination methods(alkaline potassium persulfate digestion ultraviolet spectrophotometry and ammonium molybdate spectrophotometric method)and simultaneous digestion method.The results were compared and analyzed to select suitable methods for water of different salinities.The experiment results showed that the salinity has certain interference to the determination of total nitrogen.Potassium persulfate oxidation method is suitable for the determination of water with the salinity more than 18,and the recovery rate ranged from 94.5%to 108.0%.The methods of alkaline potassium persulfate digestion UV spectrophotometry and simultaneous digestion are suitable for the determination of water with the salinity less than 4.The recovery rate of these two methods ranged from 94.0%to 108.9%.Salinity has no significant influence on the determination of total phosphorus.However,the potassium persulfate oxidation method is more accurate to determine water with salinity greater than 4 compared with other results,and the recovery rate ranged from 90.5%to 105.5%.Ammonium molybdate spectrophotometry and simultaneous digestion methods are more accurate in the detection of water with the salinity less than 12 and the recovery rate ranged from 100.9%to 107.0%.
作者
李碧莹
马晓娜
田会芹
吴乐乐
李贤
LI Bi-ying;MA Xiao-na;TIAN Hui-qin;WU Le-le;LI Xian(College of Environmental Science and Engineering,Qingdao University,Qingdao 266071,China;Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Hanzhong Rcademy of Environment Engineering Planning and Design,Hanzhong 723000,China)
出处
《海洋湖沼通报》
CSCD
北大核心
2021年第5期127-135,共9页
Transactions of Oceanology and Limnology
基金
山东省农业重大应用技术创新项目(SD2019YY011)
广东省重点研发计划(2019B020215001)
财政部和农业农村部:国家现代农业产业技术体系(CARS-47)。
关键词
水产养殖
盐度
总氮
总磷
浓度
检测方法
aquaculture
salinity
total nitrogen
total phosphorus
concentration
determination method
