摘要
白蜡树炭疽菌(Colletotrichum spaethianum)是新发现的引起龙牙百合炭疽病的病原菌,为进一步明确其生物学特性及对药剂的敏感性,本试验采用菌丝生长速率法和孢子计数法,研究不同碳源、氮源、培养基、pH、光照、温度等对白蜡树炭疽菌菌丝生长和产孢的影响;并采用菌丝生长速率法,测定该病原菌对6种杀菌剂的敏感性。结果表明,最适合白蜡树炭疽菌菌丝生长的条件:碳源为葡萄糖和乳糖,氮源为牛肉浸膏,pH值5,温度25℃,培养基为OA,12 h光照/12 h黑暗;最适产孢条件:碳源为乳糖,氮源为甘氨酸,pH值9,温度25℃,培养基为PSA,24 h黑暗;菌丝致死温度为53℃,分生孢子致死温度为58℃。参试6种杀菌剂中,吡唑醚菌酯抑制作用最强,EC_(50)为0.056 mg·L^(-1);唑醚·代森联的防治效果最差,EC_(50)为64.609 mg·L^(-1)。
Colletotrichum spaethianum is a newly discovered pathogen leading to anthracnose of‘Longya Lily’.In order to further clarify its biological characteristics and sensitivity to fungicides,this experiment adopted the mycelial growth rate method and spore counting method to study the effects of different carbon source,nitrogen source,substrate,pH,illumination,temperature,etc.on C.spaethianum mycelial growth and sporulation;This experiment also measured the sensitivities of this pathogen on 6 kinds of insecticides by mycelial growth velocity method.The results showed that the most suitable conditions for C.spaethianum mycelial growth were:carbon source-glucose source and lactose,nitrogen source-beef extract,pH value-5.0,temperature-25℃,substrate-OA,illumination-12 h light-12 h darkness;The most suitable conditions for sporulation were carbon:source-lactose,nitrogen source-glycine,pH value-9.0,temperature-25℃,substrate-PSA,24 h darkness;The mycelial lethal temperature was 53℃and conidium lethal temperature was 58℃.Among the 6 fungicides taking the test,pyraclostrobin had the strongest inhibitory effect,EC_(50) value was 0.056 mg·L^(-1);Azolether·dysene had the worst prevention and control effect,EC_(50) value was 64.609mg·L^(-1).
作者
刘佳蓉
李楠
甘韬玉
曾慧兰
李润根
LIU Jiarong;LI Nan;GAN Taoyu;ZENG Huilan;LI Rungen(Department of Horticulture,College of Life Science and Environmental Resources,Jiangxi Province Key Laboratory of Controlling and Regulating of Crop Growth and Development,Yichun University,Yichun 336000,Jiangxi,China)
出处
《中国蔬菜》
北大核心
2021年第11期75-80,共6页
China Vegetables
基金
江西省科技计划项目(20161BBF60040)
江西省教育厅科技计划项目(160132)。
关键词
百合
白蜡树炭疽菌
生物学特性
药剂敏感性
lily
Colletotrichum spaethianum
biological characteristics
sensitivity to fungicide
