摘要
目的:应用QuEChERS前处理,并评定QuEChERS-HPLC法快速测定黄豆芽中6-苄基腺嘌呤含量的不确定度。方法:通过不确定度来源分析,建立不确定度评定的数学模型,并计算各不确定度分量和合成不确定度、扩展不确定度。结果:试验中不确定度来源于标准溶液和梯度标准溶液的配制、标准曲线的拟合、试验的前处理过程、回收率和重复性以及仪器本身。果蔬类农产品(黄豆芽)中含有6-苄基腺嘌呤0.335 mg/kg;合成相对标准不确定度为0.026 mg/kg,k=2。结论:试验中不确定度的主要来源为标准曲线的拟合、梯度标准溶液的配置和回收率的测定;采用QuEChERS法能够简化前处理步骤,在减少溶剂消耗的同时降低试验的不确定度。
Objective:The uncertainty of HPLC method for determination of 6-benzyladenine in a soybean sproutswas evaluated by QuEChERS pretreatment method.Methods:A mathematical model was established by analysis of uncertainty sources.Then the individual components of uncertainty were evaluated and the combined uncertainties and expanded uncertainties were calculated.Results:The results showed that the uncertainty caused by serval factors,including the configuration of standard solutions and gradient standard solutions,the fitting of the standard curve,the pretreatment process,the recovery rate and repeatability,and the instrument itself.The content of 6-benzyladenine was 0.335 mg/kg in agricultural products(soybean sprouts).The relative combined standard uncertainty of the method was about 0.026 mg/kg,k=2.Conclusion:The main sources of uncertainty were the fitting of the standard curve,the configuration of gradient standard solutions and the recovery rate.QuEChERS method can simplify the pretreatment steps and reduce the solvent consumption and the uncertainty of the test.
作者
刘飞波
王希
刘水平
LIU Fei-bo;WANG Xi;LIU Shui-ping(Zhuzhou Institute for Food and Drug Control,Zhuzhou,Hunan 412000,China)
出处
《食品与机械》
北大核心
2021年第11期67-71,91,共6页
Food and Machinery
