摘要
目的:考察i Root BP Plus在大鼠再植磨牙牙髓血运重建的作用及对血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响。方法:以60只3周龄的雄性Wistar大鼠为研究对象,随机分为3组,每组20只,分别为空白组、对照组及研究组。建立再植牙模型后,进行牙髓血运重建术,对照组采用MTA覆盖整个血凝块面,研究组使用i Root-BP Plus,空白组不进行盖面。术后4周处死大鼠,进行影像学、免疫组化染色及VEGF基因相对表达量检测。结果:相比于空白组,对照组和研究组在影像学表现中均出现不同程度的牙根再发育,根尖增大、根尖孔变窄,感染及根尖周骨损伤范围减小,根管腔狭窄,壁增厚。其中对照组牙根尖炎症范围有一定缩小,骨组织破坏未彻底消失,根尖有异型性钙化,研究组牙根尖周炎症和骨组织破坏消失,根尖形态无明显增生及异形。相比于空白组,对照组和研究组转录因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)和碱性成纤维细胞生长因子(base fibroblast growth factor,bFGF)表达的光密度值(mean optical density,MOD)值均显著增加,相比于对照组,研究组Nrf2和bFGF表达的MOD值显著增加(P<0.05)。与空白组相比,对照组和研究组大鼠TNF-α基因相对表达量均显著降低,VEGF基因相对表达量显著升高(P<0.05);与对照组相比,研究组大鼠TNF-α基因相对表达量均显著降低,VEGF基因相对表达量显著升高(P<0.05)。结论:iRoot-BP Plus在大鼠再植磨牙血运重建术中有良好的治疗效果,能有效促进患牙愈合。
Objective:To investigate the effect of i Root BP Plus on the pulp revascularization of the replanted molars in rats and its influence on VEGF.Methods:Taking 60 male Wistar rats aged 3 weeks as the research object,they were randomly divided into 3 groups with 20 rats in each group,namely the blank group,the control group and the study group.After the reimplantation model was established,pulp revascularization was performed.The control group used MTA to cover the entire clot surface,the study group used i Root-BP Plus,and the blank group did not cover the surface.The rats were sacrificed 4 weeks after the operation,and imaging,immunohistochemical staining and relative expression of VEGF gene were detected.Results:Compared with the blank group,both the control group and the study group had different degrees of root redevelopment,the root apex enlarged,the apical foramen narrowed,the scope of infection and periapical bone injury decreased,the root canal cavity was narrowed,and the wall thickened.In the control group,the range of apical inflammation was reduced,the destruction of bone tissue did not completely disappear,and there was atypical calcification in the apex of the tooth.In the study group,inflammation and destruction of bone tissue disappeared,and there was no obvious proliferation and abnormal shape of the apical morphology.Compared with the blank group,the MOD value of Nrf2 and bFGF expression in the control group and the study group increased significantly.Compared with the control group,the MOD value of Nrf2 and b FGF expression in the study group increased significantly(P<0.05).Compared with the blank group,the relative expression of TNF-αgene in the control group and the study group were significantly reduced,and the relative expression of VEGF gene was significantly increased(P<0.05);Compared with the control group,the relative expression of TNF-αgene in the study group was significantly reduced,and the relative expression of VEGF gene was significantly increased(P<0.05).Conclusion:IRoot-BP Plus has a good therapeutic effect in the revascularization of replanted molars in rats,and can effectively promote the healing of the affected teeth.
作者
马丽
柴娟
高陶磊
李丽霞
李洋
王健
MA Li;CHAI Juan;GAO Tao-lei;LI Li-xia;LI Yang;WANG Jian(Department of Stomatology,Guangren Hospital Affiliated to Xi'an Jiaotong University(Xi'an Fourth Hospital),Xi'an,Shaanxi,710004,China;School of Stomatology,Xi'an Medical College,Xi'an,Shaanxi,710021,China;General Department,Hospital of Stomatology,Xi'an Jiaotong University,Xi'an,Shaanxi,710004,China;Department of Stomatology,Xi'an First Hospital,Xi'an,Shaanxi,710002,China)
出处
《现代生物医学进展》
CAS
2021年第19期3645-3648,3664,共5页
Progress in Modern Biomedicine
基金
陕西省自然科学基础研究计划项目(2019JQ-886)。
