摘要
为了了解2019—2020年广东省猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)的遗传变异情况,试验采用RT-PCR方法对采集到的331份疑似感染PRRSV的临床样品进行病原检测,并对能同时检测到GP5和NSP2基因的阳性样品进行测序,再通过分子生物学软件将测序结果与GenBank中的参考毒株进行遗传进化和核苷酸序列同源性分析。结果表明:检测的331份样品中有65份呈阳性,阳性率为19.6%,PRRSV在广东省具有较高的感染率。对能同时检测出GP5和NSP2基因的阳性样品进行测序,最终获得11株PRRSV的GP5和NSP2基因序列。对11株毒株的GP5基因进行遗传进化分析,有3株属于Lineage 8.7(JXA1和CH-1a)亚群,1株属于Lineage 5.1亚群,3株属于Lineage 1(NADC30)亚群,4株属于以QYYZ为代表的Lineage 3亚群,11株PRRSV毒株的GP5基因核苷酸序列同源性为78.9%~99.2%。对11株PRRSV毒株的NSP2基因进行遗传进化分析,有3株属于以JXA1毒株为代表的Lineage 8.7亚群,1株属于以QYYZ毒株为代表的Lineage 3亚群,2株属于以欧洲型代表株LV为代表的亚群,5株属于以NADC30为代表的Lineage 1亚群,11株PRRSV毒株的NSP2基因核苷酸序列同源性为32.1%~98.4%。说明2019—2020年广东省的PRRSV主要流行毒株属于Lineage 3、Lineage 1(NADC30)和Lineage 8.7(JXA1和CH-1a)亚群。
In order to understand the genetic variation of Porcine reproductive and respiratory syndrome virus(PRRSV) in Guangdong Province from 2019 to 2020, in this study, 331 samples suspected to be infected with PRRSV were collected for antigen detection by RT-PCR. The GP5 and NSP2 gens of some positive samples were sequenced, and then used molecular biology software to analyze the genetic evolution tree and nucleotide sequence homology between the sequencing results and the reference strains in GenBank. The results showed that 65 samples were positive, with a positive rate of 19.6%. PRRSV had a high incidence in Guangdong Province. The GP5 and NSP2 gene sequences of 11 PRRSV strains were obtained by sequencing the GP5 and NSP2 gene sequences. The genetic evolution analysis of the GP5 gene of 11 strains showed that three strains belonged to the Lineage 8.7(JXA1 and CH-1 a) subgroup, one strain belonged to the Lineage 5.1 subgroup, three strains belonged to the Lineage 1(NADC30) subgroup, and four strains It belongs to the Lineage 3 subgroup represented by QYYZ. The nucleotide sequence homology of GP5 gene of 11 strains was 78.9%-99.2%. The genetic evolution analysis of the NSP2 genes of 11 strains showed that three strains belonged to the Lineage 8.7 subgroup represented by the JXA1 strain, one strain belonged to the Lineage 3 subgroup represented by the QYYZ strain, and two strains belonged to the European type Representative strain LV represents the subgroup, five strains belong to the Lineage 1 subgroup represented by NADC30. The nucleotide sequence homology of the NSP2 gene of 11 strains is 32.1%-98.4%. It indicated that the main epidemic strains of PRRS in Guangdong Province from 2019 to 2020 were located in the Lineage 3, Lineage 1(NADC30) and Lineage 8.7(JXA1 and CH-1 a) subgroups.
作者
王爽云
于林洋
梁太润
董建国
杨霞
刘燕玲
张乐宜
宋长绪
WANG Shuangyun;YU Linyang;LIANG Tairun;DONG Jianguo;YANG Xia;LIU Yanling;ZHANG Leyi;SONG Changxu(College of Animal Science,South China Agricultural University,Guangzhou 510642,China;College of Animal Husbandry Medicine Engineering,Xinyang Agriculture and Forestry University,Xinyang 464000,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第21期15-22,共8页
Heilongjiang Animal Science And veterinary Medicine
