摘要
【目的】研究臭氧(O_(3))胁迫下白皮松的生长、生理和吸收特性的变化,阐释各指标间的相关关系,确定其对O_(3)的响应程度。【方法】以白皮松幼苗为试验对象,设置了3个浓度梯度(NF:正常环境大气O_(3)浓度,NF40:正常大气环境O_(3)浓度加40 nmlol/mol;NF80:正常大气环境O_(3)浓度加80 nmol/mol)的开顶式O_(3)熏蒸人工控制试验,测定其生长状况、生理特性以及O_(3)吸收能力,通过相关性分析、冗余分析和方差分析对不同O_(3)浓度下白皮松各特性之间的关系进行研究。【结果】1)白皮松的株高增长(△H)、50 cm处直径增长(DDBH)、气孔大小(S)、气孔密度(M)、气孔开度(K)、气孔导度(G_(s))、净光合速率(P_(n))、蒸腾速率(Et)、水分利用率(WUE)、最大光化学效率(F_(v)/F_(m))、叶绿素含量(CHL)、整树耗水量(W)和O_(3)吸收速率(FO_(3))均随着O_(3)浓度的升高而降低;而胞间CO_(2)浓度(Ci)和相对电导率(L)则随着O_(3)浓度的升高而升高;2)O_(3)胁迫下白皮松生长指标(DH、DDBH)与O_(3)吸收状况(FO_(3)、W)相关性最高,其次是光合指标(Pn、WUE、Et、G_(s)、Ci)与生长指标(△H、△DBH)和气孔特性(K、M、S),部分生理指标(L、Fv/Fm)与光合(Pn、WUE、E_(t)、G_(s)、C_(i))和气孔(K、M、S)的相关性相对较弱;3)白皮松的各项指标在NF和NF80的O_(3)处理下差异均显著(P<0.05),△H、△DBH、M、CHL、P_(n)、G_(s)、W和FO_(3)在NF和NF40处理下差异最显著,K、S、WUE、F_(v)/F_(m)、E_(t)、C_(i)、L在NF40和NF80处理下差异更显著。【结论】试验证明长期处于高浓度的O_(3)环境中,白皮松的生长缓慢,光合能力下降,对O_(3)的吸收能力也进一步降低,白皮松的生长与O_(3)吸收特性变化相关性最高,气孔特性与光合生理特性相关性最高,而光合能力等的下降又进一步导致其生长受到遏制。
【Objective】The changes of growth,physiology and uptake characteristics of Pinus bungeana under ozone(O_(3))stress were studied,and the correlations among various indexes were explained,which defined the response of Pinus bungeana seedlings to O_(3).【Method】Three O_(3) exposure environments were set for Pinus bungeana seedlings(NF:normal atmospheric O_(3) concentration;NF40:normal atmospheric O_(3) concentration plus 40 nmol/mol;NF80:normal atmospheric O_(3) concentration plus 80 nmol/mol).The artificial control experiment of open-top O_(3) fumigation was carried out to determine the growth status,physiological characteristics and O_(3) uptake capacity of Pinus bungeana,and then the correlations of characteristics under different O_(3) concentrations were studied by correlation analysis,redundancy analysis and variance analysis.【Result】1)With the increase of O_(3) concentration,plant height increment(△H),diameter variation at a height of 50 cm(△DBH),stomatal size(S),stomatal density(M),stomatal opening(K),stomatal conductance(G_(s)),net photosynthetic rate(P_(n)),transpiration rate(Et),water use efficiency(WUE),maximum photochemical efficiency(Fv/Fm),chlorophyll content(CHL),whole tree water consumption(W)and O_(3) uptake rate(FO_(3))decreased,while the intercellular CO2 concentration(C_(i))and relative conductivity(L)increased.2)Under O_(3) stress,correlation degree of growth indexes(△H,△DBH)and O_(3) uptake indexes(FO_(3),W)was great,which was followed by the correlation degree of photosynthetic indexes(P_(n),WUE,E_(t),G_(s),C_(i)),growth indexes(△H,△DBH)and stomatal characteristics(K,M,S),while some physiological indexes(L,Fv/Fm)had relatively weak correlations with photosynthesis indexes(P_(n),WUE,E_(t),G_(s),C_(i))and stomata indexes(K,M,S).3)All indexes of Pinus bungeana were significantly different under O_(3) treatment of NF and NF80(P<0.05).△H,△DBH,M,CHL,P_(n),G_(s),W and FO_(3) differed the most in NF and NF40,but K,S,WUE,Fv/Fm,Et,Ci and L were more significantly different in NF40 and NF80.【Conclusion】It showed that long-term exposure to high O_(3) concentration results in slow growth,photosynthetic capacity decrease and O_(3) uptake capacity decrease of Pinus bungeana.The growth of Pinus bungeana has great correlation with the change of O_(3) uptake,and the stomatal characteristics have great correlation with photosynthetic physiological characteristics.Furturemore,the decline of photosynthetic capacity further suppresses seedlings growth.
作者
徐晶晶
刘鹏
郑书旗
陈波
杨新兵
XU Jingjing;LIU Peng;ZHENG Shuqi;CHEN Bo;YANG Xinbing(College of Forestry,Hebei Agriculture University,Baoding 071001,Hebei,China;Beijing Academy of Forestry and Pomology Sciences,Beijing 100093,China;Beijing World Hazard Preventing Tech.Co.Ltd,Beijing 100048,China)
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2021年第11期56-64,72,共10页
Journal of Central South University of Forestry & Technology
基金
国家自然科学基金项目(31500352)
北京市林业果树科学研究院院长基金项目(201903)
北京市农林科学院青年基金项目(QNJJ202017)。
