摘要
目的研究血塞通(主要成分三七皂苷,PNS)治疗哮喘小鼠气道炎症的免疫分子机制。方法血塞通软胶囊三七总皂苷含量标定质量浓度为100 mg·mL^(-1)的溶液,将50只SPF级雌性巴比西(BALB/c)小鼠随机分为5个组:空白组、模型组和低、中、高剂量实验组。在第0、7、14天,对空白组小鼠腹腔内注射生理盐水造模,模型组和低、中、高剂量实验组小鼠腹腔内注射卵清蛋白(OVA)致敏。第15天空白组用生理盐水进行雾化激发,模型组和低、中、高剂量实验组小鼠使用OVA溶液雾化激发造模。分别在激发前1 h对低、中、高剂量实验组小鼠腹腔注射血塞通溶液200μL(浓度标定为PNS浓度0.5,1.5,3.0 mg·μL^(-1)),空白组与对照组注射等量纯净水。分析小鼠肺泡灌洗液细胞,检测外周血白细胞介素-10(IL-10)和白细胞介素-17(IL-17)的水平,用苏木精-伊红(HE)染色观察小鼠肺组织进行病理切片,以流式细胞术检测TH17细胞比例。结果空白组、模型组和低、中和高剂量实验组的小鼠外周血中IL-10分别为(89.21±13.24),(35.66±9.87),(43.12±9.18),(59.84±11.38),(72.45±13.01)pg·mL^(-1);IL-17分别为(120.16±8.63),(250.13±18.16),(210.96±9.79),(176.32±13.56),(140.55±16.89)pg·mL^(-1);小鼠外周血中Th-17的细胞比例分别为(1.52±1.24)%,(8.03±1.52)%,(5.63±1.79)%,(3.02±1.33)%,(1.99±1.81)%。低、中和高剂量实验组的上述指标与模型组相比,差异均有统计学意义,并呈现浓度依赖(均P<0.05)。通过观察小鼠肺组织HE染色,低、中、高剂量实验组与模型组相比,小鼠气道周围炎症细胞浸润减轻,支气管壁变薄及黏液腺减少。结论PNS能增加IL-10的分泌,减少IL-17的分泌,减少Th-17的细胞比例,同时明显改善小鼠气道周围炎症细胞浸润程度、支气管壁增厚及黏液腺增生等现象。
Objective To investigate the immunomolecular mechanisms of airway inflammation in mice with asthma treated with Panax notoginseng saponin(Xuesaitong).Methods The solution of total saponin content of Xuesaitong soft capsule calibrated to a mass concentration of 100 mg·mL^(-1),50 SPF-grade female BALB/c mice were randomly divided into 5 groups:blank group,model group and low,medium,high dose experimental groups,and the mice in blank group were injected intraperitoneally with normal saline at 0,7 and 14 d to create a model,and the mice in model group and low,medium,high dose experimental groups were sensitized by intraperitoneal injection of ova lb umin(OVA)sensitizing solution.On the 15th day,the blank group was stimulated by nebulization with normal saline,model group and low,medium,high dose experimental groups were stimulated by nebulization with OVA solution.The mice in low,medium,high dose experimental groups were injected intraperitoneally with Xuesaitong solution 200μL at 1 hour before excitation(the dose of panax notoginseng saponin was calibrated to 0.5,1.5 and 3.0 mg·μL^(-1)),and the mice in model group and blank group were injected intraperitoneally with purified water.The cells in alveolar lavage fluid of mice were analyzed,the concentration levels of interleukin-10(IL-10)and interleukin-17(IL-17)in peripheral blood were detected,the lung tissues of mice were observed by HE staining in pathological sections,and the ratio of Th-17 cells was detected by flow cytometry.Results The amount of IL-10 in peripheral blood in blank group,model group and low,medium,high dose experimental groups were(89.21±13.24),(35.66±9.87),(43.12±9.18),(59.84±11.38),(72.45±13.01)pg·mL^(-1);the amount of IL-17 were(120.16±8.63),(250.13±18.16),(210.96±9.79),(176.32±13.56),(140.55±16.89)pg·mL^(-1);the proportion of Th-17 cells in the peripheral blood of mice were(1.52±1.24)%,(8.03±1.52)%,(5.63±1.79)%,(3.02±1.33)%,(1.99±1.81)%.Compared with model group,the above indicators in low,medium,high dose experimental groups were statistically different,and with concentration-dependent(all P<0.05).By observing the HE staining of mouse lung tissue,the low,medium and high dose experimental groups compared with model group,the airway infiltration of inflammatory cells reduced,bronchial wall thinning and mucous glands reduce.Conclusion Panax ginseng saponin increased the secretion of IL-10,decreased the secretion of IL-17 and reduced the proportion of Th-17 cells,while the degree of inflammatory cell infiltration around the airways,bronchial wall thickening and mucus gland hyperplasia in mice were improved significantly.
作者
关海燕
鲁娜
GUAN Hai-yan;LU Na(Department of Pediatrics,Wuhan East-West Lake District People’s Hospital,Wuhan 430050,Hubei Province,China;Department of Pediatrics,Wuhan Union Hospital,Wuhan 430050,Hubei Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2021年第22期3102-3106,共5页
The Chinese Journal of Clinical Pharmacology
关键词
血塞通软胶囊
三七皂苷
哮喘
气道炎症
免疫分子机制
Xuesaitong soft capsule
Panax notoginseng saponin
asthma
airway inflammation
immune molecular mechanism
