摘要
目的探究过表达miR-126对增生性玻璃体视网膜病变大鼠的干预作用及相关机制。方法选取40只SD健康雄性大鼠,10只为正常组,其余30只建立增生性玻璃体视网膜病变模型,并分为模型组、miR-126沉默组、miR-126过表达组,每组10只,4组大鼠分别干预。比较4组大鼠血流动力学水平[收缩期峰值血流速度(Max)、舒张末期流速(Min)、平均血流速度(TAMx)],RT-PCR检测miR-126、VEGF mRNA表达,酶联免疫吸附实验法检测[超敏C-反应蛋白(hs-CRP)、白介素-17(IL-17)、转化生长因子β1(TGF-β1)]水平,检测4组大鼠T淋巴细胞亚群水平,蛋白质印迹法检测E-cadherin、vimentin、snail表达。结果与模型组、miR-126沉默组比较,miR-126过表达组大鼠miR-126、E-cadherin表达较高,VEGF mRNA、vimentin、snail表达较低(P<0.05)。与模型组、miR-126沉默组比较,miR-126过表达组大鼠Max、Min、TAMx水平较高(P<0.05)。miR-126沉默组大鼠hs-CRP、IL-17、TGF-β1水平高于模型组,且miR-126过表达组大鼠hs-CRP、IL-17、TGF-β1、CD_(8)^(+)水平低于模型组、miR-126沉默组,CD_(4)^(+)、CD_(3)^(+)水平高于模型组、miR-126沉默组,差异有统计学意义(P<0.05)。结论上调增生性玻璃体视网膜病变大鼠miR-126表达,能够靶向调控VEGF mRNA,抑制大鼠视网膜新生血管形成,改善血流动力学水平,减轻炎性反应,提升免疫功能,阻断视网膜上皮细胞上皮-间充质转化,为增生性玻璃体视网膜病变的临床治疗提供一定的参考依据。
Objective To investigate the intervention effects and related mechanism of miR 126 over-expression in rats with proliferative vitreoretinopathy.Methods A total of 40 SD healthy male rats were enrolled in the study,of whom,10 rats were enrolled as control group,and the rats models with proliferative vitreoretinopathy were established in the other 30 rats,who were divided into model group,miR-126 silencing group,and miR-126 over-expression group,with 10 rats in each group.The rats in the four groups were intervened,respectively.The hemodynamic indexes including Max,Min,TAMx were detected.The expression levels of miR-126 and VEGF mRNA were detected by RT-PCR,and the levels of hs-CRP,IL-17 and TGF-β1 were measured by enzyme linked immunosorbent assay,and the levels of T lymphocyte subsets were determined,moreover the expression levels of E cadherin,vimentin and snail were detected by Western Blot.Results As compared with those in model group and miR-126 silencing group,the expression levels of miR-126 and E cadherinin miR over-expression group were significantly increased,however,the expression levels of VEGF mRNA,vimentin and snail were significantly decreased(P<0.05).As compared with those in model group and miR-126 silencing group.The levels of Max,Min and TAMx in miR-126 over-expression group were significantly increased(P<0.05).The levels of hs-CRP,IL-17,TGF-β1 in miR-126 silencing group were significantly higher than those in model group,however,the levels of hs-CRP,IL-17,TGF-β1 and CD_(8)^(+) were significantly lower than those in model group and miR-126 silencing group(P<0.05).Conclusion The upregulation of miR-126 expression in rats with proliferative vitreoretinopathy can target and regulate VEGF mRNA,inhibit retinal neovascularization,improve hemodynamic level,alleviate inflammation reaction,improve immune function,block the epithelial mesenchymal transition of retinal epithelial cells,whichi provides certain reference for clinical treatment of proliferative vitreoretinopathy.
作者
徐智科
李臻
蒲一民
XU Zhike;LI Zhen;PU Yimin(Department of Ophthalmology,Leshan People’s Hospital,Sichuang,Leshan 614000,China)
出处
《河北医药》
CAS
2021年第23期3535-3538,3543,共5页
Hebei Medical Journal
基金
四川省卫生和计划生育委员会科研课题(编号:17PJ548)。
关键词
增生性玻璃体视网膜病变
炎性反应
免疫功能
血流动力学
上皮-间充质转化
proliferative vitreoretinopathy
inflammatory reaction
immunological functioning
hemodynamics
epithelial mesenchymal transition
