摘要
目的探讨BALB/c突变卷毛鼠与正常BALB/c小鼠构建经肝门静脉注射肿瘤模型间的差异,同时建立基于Image J软件的肿瘤图像定量分析方法。方法将H_(22)细胞分别经两组小鼠的肝门静脉注入肝,21 d后摘取肝。选择最大肝叶弥散病灶,首先拍照获得垂直视角肿瘤外观图进行比较,然后利用Image J软件对肝肿瘤进行图像定量分析比较。结果两组小鼠的肝均形成了多发性弥散性肿瘤。外观显示:卷毛鼠组肿瘤的多发和弥散程度显著高于正常小鼠组,且肿瘤与周边的正常肝组织浸润关系更加密切。Image J软件分析结果显示两组小鼠肝比例之间不具有显著性差异,但卷毛鼠的肿瘤比例和肿瘤占肝的比例均显著大于正常小鼠(P<0.01)。结论成功模拟了进展期肝肿瘤动物模型。BALB/c突变卷毛鼠在经门静脉注射肿瘤细胞成模能力显著优于正常BALB/c小鼠,同时建立了基于图像分析软件Image J的图像定量分析方法。
Objective To investigate the difference between BALB/c mutant curly mice and normal BALB/c mice in the establishment of tumor models by hepatic portal vein injection and establish a quantitative analysis method of images using Image J software. Methods H_(22)cells were injected into the liver through the portal vein of the two groups and livers were removed 21 days later. The largest diffuse lesion of the liver lobe was selected for analysis. The vertical view of the tumor appearance was obtained by acquiring images, and then Image J software was used for quantitative analysis and comparison of liver tumor images. Results Multiple diffuse tumors were found in both groups. The multiple and diffuse degree of tumors in the curly mice group was significantly higher than that in the normal mice group, and the tumors in curly mice group were more closely related to the surrounding normal liver tissue infiltration than that in normal mice group. The result of Image J software analysis showed that there was no significant difference between the two groups, but the proportions of tumors and tumors in the liver of curly mice were significantly higher than those in normal mice(P<0.01). Conclusions The animal model of advanced liver tumors was established successfully. The ability of BALB/c mutant curly mice to model tumor cells via portal vein injection was significantly better than that of normal BALB/c mice. Moreover, a quantitative analysis method of images was established using Image J software.
作者
李晓娟
李兴杰
孙慧伟
姜棋予
柴燕涛
高蓉
李润
李瑞生
汤紫荣
LI Xiaojuan;LI Xingjie;SUN Huiwei;JIANG Qiyu;CHAI Yantao;GAO Rong;LI Run;LI Ruisheng;TANG Zirong(Department of Infectious Disease medicine,Fifth Medical Center of Chinese PLA General Hospital,Beijing 100039,China;Inpatient and Medical Record Management Department,Fifth Medical Center of Chinese PLA General Hospital,Beijing 100039)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第11期16-20,共5页
Chinese Journal of Comparative Medicine
基金
军队实验动物专项基金面上项目(SYDW[2016]005,SYDW[2020]05)。
关键词
卷毛鼠
肝进展期肿瘤模型
图像分析
curly hair mice
liver advanced tumor model
image analysis
