摘要
为了解贝氏柯克斯体伴随蜱活动的流行特征以及该病原的分子特征,采用布旗法收集黑龙江省伊春、鹤岗及佳木斯等地共计461份饥饿蜱,并通过形态学鉴定其种类。经PCR扩增病原的16S rRNA基因,并测序,分析统计不同蜱种感染贝氏柯克斯体阳性率。采用Neighbor-Joining方法构建遗传进化树,分析不同地域不同蜱种来源贝氏柯克斯体的遗传进化关系。形态学鉴定结果表明,收集的蜱种主要包括日本血蜱(n=102)、全沟硬蜱(n=97)、森林革蜱(n=150)以及嗜群血蜱(n=112)。其中,日本血蜱的贝氏柯克斯体感染率高达12%,嗜群血蜱的贝氏柯克斯体感染率为6%,而全沟硬蜱及森林革蜱的贝氏柯克斯体感染率均为4%。基于16S rRNA序列分析表明,贝氏柯克斯体是一类高度保守的病原,不同地方株之间序列差异不显著。且贝氏柯克斯体与多个变形菌有着较近的亲缘关系,特别是G-变形菌在长期的进化过程中,有一支分化为贝氏柯克斯体,另外一支依然保持了G-变形菌的分子特征。基于上述分析,贝氏柯克斯体作为一种重要的人兽共患病原,可在多种蜱体内广泛存在,但其感染率略有不同。该病原的存在会给当地家畜及人的活动造成潜在的危害,为该病原的防控提出了挑战。因此,加强当地家畜饲养管理将成为首要解决的问题。其次,杀蜱也是防控Q热病原极其有效的方法。
In order to clarify the relationship of the epidemic characteristics of Coxiella burnetii associate with the activities of ticks,and to know the molecular characteristics of the pathogen,461 ticks were collected by flag laying method from Yichun,Hegang and Jiamusi in Heilongjiang Province,China.The 16S rRNA gene sequence of the pathogens in various ticks was amplified by PCR and sequenced.The positive rate of C.burnetii was analyzed in different tick species.The phylogenetic tree based on 16S rRNA of C.burnetii from different regions was constructed by Neighbor-Joining method.The results of morphological identification showed that the collected ticks mainly included Haemaphysalis japonica(n=102),Ixodes persulcatus(n=97),Dermacentor silvarum(n=150),H.concinna(n=112).The PCR results showed that the infection rate of C.burnetii was 12%in H.japonica and 6%in H.concinna.The infection rates of C.burnetii in I.persulcatus and D.silvarum were both 4%.Based on16 S r RNA sequence analysis,C.burnetii was a highly conserved pathogen,and there was no significant sequence difference among different strains.In particular,during the long-term evolution of G-proteobacteria,one branch differentiated into C.burnetii,and the other branch still maintained the molecular characteristics of G-proteobacteria.Based on the aboved results,C.burnetii as an important zoonotic pathogen,is widely exists in different tick speciess,but its infection rate is not high.But the existence of this pathogen is still a potential risk to local livestock and human health,which poses a challenge for the prevention and control of the pathogen.Therefore,strengthening the local livestock management will become the first work to perform.Secondly,killing ticks is also an effective way to control Q fever pathogen.
作者
罗金
李静
张高峰
刘佩雯
罗文蔚
苏小玲
任巧云
刘文阁
曹润来
刁培文
孙世弟
关贵全
罗建勋
殷宏
李祥瑞
刘光远
LUO Jin;LI Jing;ZHANG Gao-feng;LIU Pei-wen;LUO Wen-wei;SU Xiao-ling;REN Qiao-yun;LIU Wen-ge;CAO Run-lai;DIAO Pei-wen;SUN Shi-di;GUAN Gui-quan;LUO Jian-xun;YIN Hong;LI Xiang-rui;LIU Guang-yuan(State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province/Key Open Laboratory of Herbivores Animal Epidemics,Ministry of Agriculture/Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China;MOE Joint International Research Laboratory of Animal Health and Food Safety/College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;AnimalDisease Prevention and Control Center of Qinghai Province,Xining 810000,China;China Agricultural Vet.Bio.Science and Technology CO.,LTD.,Lanzhou 730046,China;Lanzhou New Area Bureau of Agriculture,Forestry and Irrigation,Lanzhou 730300,China;College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2021年第11期1385-1391,共7页
Chinese Veterinary Science
基金
国家重点研发计划项目(2019YFC1200502,2019YFC1200500)
国家寄生虫资源中心项目(NPRC-2019-194-30)
国家肉牛牦牛产业技术体系项目(CARS-37)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-LVRI)。
关键词
蜱
贝氏柯克斯体
Q热
流行病学调查
tick
Coxiella burnetii
Q fever
epidemiological investigation
