摘要
采用超临界CO_(2)萃取的方法提取得到陈皮精油和茶叶精油,并对其化学成分进行分析。选用以α-对硝基苯酚葡萄糖苷(PNPG)为底物的筛选模型,对陈皮精油和茶油进行α-葡萄糖苷酶的抑制活性测试,并以阿卡波糖作为阳性对照作抑制曲线。测试结果显示,陈皮精油和茶叶精油的IC_(50)分别是30.53μg/mL和118.48μg/mL。此外,还对陈皮精油和茶叶精油联合抑制作用进行研究,结果表明:当两者的重量比为11时,其对α-葡萄糖苷酶起协同抑制的作用。研究结果显示陈皮精油和茶油的联合用药能够有效抑制α-葡萄糖苷酶的活性,为高效筛选潜在降糖药物提供新思路,对天然植物精油的降糖作用研究奠定了坚实的基础。
In this study,we firstly extracted essential oils of tangerine peel and tea by supercritical CO_(2) extraction and analyzed the main constituents of these essential oils.Secondly,the screening model withα--nitrophenol glucoside(PNPG)used the inhibitory activity test of tangerine peel essential oil and tea oil,and used acboose as the positive control as the inhibition curve.The results showed that the IC_(50) of tangerine peel and tea essential oils were 30.53μg/mL and 118.48μg/mL,respectively.In addition,we studied the combined inhibition of tangerine and tea oils with a weight ratio of 11.These results demonstrated that the combination of tangerine peel essential oil and tea oil could effectively inhibit the activity ofα-glucosidase,providing new ideas for the efficient screening of potential hypoglycemic drugs,and lay a solid foundation for the study of natural plant essential oils.
作者
李永莲
游宗霖
鄢嘉炜
侯欣桦
叶秀雅
YU Yufeng
ZHANG Yangbing
LI Yonglian;YOU Zonglin;YAN Jiawei;HOU Xinhua;YU Yufeng;ZHANG Yangbing;YE Xiuya(Guangdong Industry Polytechnic,Guangzhou 510300,China;School of Biotechnology and Health Sciences,Wuyi University,Jiangmen 529020,China)
出处
《广东轻工职业技术学院学报》
2021年第4期1-5,共5页
Journal of Guangdong Industry Polytechnic
基金
广东省高校青年创新人才项目(自然科学类)(2017GkQNCX006)
2018年广东轻工职业技术学院优秀青年基金项目(QN2018-005)
广东轻工职业技术学院第二十届“挑战杯”大学生课外学术科技作品竞赛立项项目。
关键词
植物精油
Α-葡萄糖苷酶
协同作用
plant essential oil
α-glucosidase
synergistic inhibitory effect
