摘要
为探究H_(2)S缓解小麦干旱胁迫的作用机制,以20%聚乙二醇6000(PEG-6000)模拟中度干旱胁迫处理水培百农207小麦幼苗,以1 mmol/L NaHS为H_(2)S供体对小麦幼苗进行叶面喷施处理,研究H_(2)S对干旱胁迫小麦幼苗光合指标的影响。结果表明,干旱胁迫条件下,与未喷施NaHS的干旱处理组相比,外源喷施NaHS可显著缓解干旱胁迫对小麦幼苗生长及叶片光合作用的抑制效应,具体表现为小麦幼苗单株鲜质量及干质量分别显著增加21.77%和13.63%;根系相对含水量及含水量显著增加8.72%和1.89%;叶绿素、叶绿素a、叶绿素b和类胡萝卜素含量分别显著增加13.33%、25.59%、51.03%和25.92%;叶绿素荧光指标Fv/Fo、Fv/Fm、Eto/RC、PhiE0及PI_(abs)显著增加了13.47%、2.70%、23.85%、43.25%和100.78%,叶绿素荧光指标ABS/RC、TRo/RC及PhiD0显著降低了17.25%、11.34%和9.46%。干旱胁迫下外源叶面喷施H_(2)S提升了小麦幼苗的生长及光合作用。
To explore the mechanism of H_(2)S alleviating the drought stress of wheat,20%polyethylene glycol 6000(PEG-6000)was used to simulate the moderate drought stress treatment of hydroponic Bainong 207 wheat seedlings,and 1 mmol/L NaHS was used as the H_(2)S donor to spray the wheat seedlings to study the effect of H_(2)S on photosynthetic indexes of wheat seedlings under drought stress.The results showed that compared with no NaHS treatment,NaHS could significantly alleviate the inhibitory effect of drought stress on wheat seedling growth and leaf photosynthesis.The result showed that the fresh quality and dry quality of wheat seedlings increased by 21.77%and 13.63%,respectively.The relative water content and water content of the root system increased by 8.72%and 1.89%,respectively.The content of chlorophyll,chlorophyll a,chlorophyll b and carotenoid significantly increased by 13.33%,25.59%,51.03%and 25.92%,respectively.Chlorophyll fluorescence indicators Fv/Fo,Fv/Fm,Eto/RC,PhiE0 and PI_(abs) increased significantly by 13.47%,2.70%,23.85%,43.25%and 100.78%,respectively,the chlorophyll fluorescence indicators ABS/RC,TRo/RC and PhiD0 significantly reduced by 17.25%,11.34%and 9.46%,respectively.Exogenous spraying of H_(2)S on leaves under drought stress improved the growth and photosynthesis of wheat seedlings.
作者
阚文杰
席金凤
姚缘圆
苏鹏飞
曹明辉
袁梦
李明浩
汤才国
吴丽芳
KAN Wenjie;XI Jinfeng;YAO Yuanyuan;SU Pengfei;CAO Minghui;YUAN Meng;LI Minghao;TANG Caiguo;WU Lifang(The Center for Ion Beam Bioengineering&Green Agriculture,Hefei Institutes of Physical Science,Chinese Academy ofSciences,Hefei 230031,China;Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China;Zhongke Taihe Experimental Station,Taihe 236626,China)
出处
《山西农业科学》
2021年第12期1419-1425,共7页
Journal of Shanxi Agricultural Sciences
基金
安徽省科技重大专项(202103a06020002)
中国科学院合肥物质科学研究院院长基金青年“火花”项目(YZJJ2020QN29)。
关键词
小麦
干旱
光合特性
叶绿素荧光
硫化氢
wheat
drought
photosynthetic characteristics
chlorophyll fluorescence
hydrogen sulfide
