摘要
目的观察左氧氟沙星和莫西沙星治疗豚鼠实验感染鼠疫的效果。方法选择SPF级豚鼠70只,雌性,体重为250~300 g,按体重采用随机数字表法分为7组,每组10只,分别为左氧氟沙星24、48、72 h组,莫西沙星24、48、72 h组(开始给药时间点分别为感染鼠疫菌24、48、72 h)和对照组(未进行治疗)。各组豚鼠皮下注射鼠疫菌141株[1×107菌落形成单位(CFU)菌悬液]制备实验感染鼠疫模型,参照《中华人民共和国国家药典》成人剂量,用动物折算系数法换算成豚鼠每日用药剂量进行治疗,每只豚鼠左氧氟沙星、莫西沙星给药量分别为12.0、9.6 mg/d,连续用药9 d。停药观察9 d后处死豚鼠,进行细菌学检验和病理学观察,并计算治愈率。结果左氧氟沙星24、48、72 h组治愈率分别为100.0%(10/10)、70.0%(7/10)、6/6,莫西沙星24、48、72 h组治愈率分别为100.0%(10/10)、100.0%(10/10)、5/7,对照组治愈率为0(0/10)。与对照组比较,左氧氟沙星24、48、72 h组和莫西沙星24、48、72 h组治愈率均明显较高(P均<0.05);相同开始给药时间点的两种药品间治愈率比较,差异均无统计学意义(P均>0.05)。结论左氧氟沙星和莫西沙星治疗豚鼠实验感染鼠疫的效果理想,特殊情况下可用于鼠疫治疗。
Objective To observe the efficacy of levofloxacin and moxifloxacin in the treatment of experimental plague in guinea pigs.Methods A total of 70 SPF guinea pigs,female,weighing 250 to 300 g,were selected and randomly divided into 7 groups according to body weight by random number table.There were 10 guinea pigs in each group:levofloxacin 24,48,72 h groups,moxifloxacin 24,48,72 h groups(drug treatment was carried out after being infected with Yersinia pestis for 24,48 and 72 h,respectively)and control group(without treatment).Experimentally infected plague model was established through guinea pigs subcutaneous injection of 141 strains of Yersinia pestis[1×107 colony forming unit(CFU)];referring to the adult dose in the"National Pharmacopoeia of the People's Republic of China",the daily dose of guinea pigs was converted by Shi Xinyou's animal conversion coefficient method for treatment,the doses of levofloxacin and moxifloxacin in each guinea pig were 12.0 and 9.6 mg/d,respectively for 9 days.The guinea pigs were killed 9 days after drug withdrawal for bacteriological examination and pathological observation,and the cure rate was calculated.Results The cure rates of levofloxacin 24,48 and 72 h groups were 100.0%(10/10),70.0%(7/10)and 6/6,respectively.The cure rates of moxifloxacin 24,48 and 72 h groups were 100.0%(10/10),100.0%(10/10)and 5/7,respectively,and the cure rate of animals in the control group was 0(0/10).Compared with the control group,there were significant differences in the efficacy of moxifloxacin 24,48 and 72 h groups and levofloxacin 24,48 and 72 h groups(P<0.05).There was no significant difference between the two drugs at the same starting time(P>0.05).Conclusion The effects of levofloxacin and moxifloxacin on animal plague infection are ideal and the two drugs can be used as a substitute for streptomycin in plague treatment under special circumstances.
作者
何建
辛有全
杨晓艳
李胜
张琪
靳娟
杨建国
熊浩明
代瑞霞
He Jian;Xin Youquan;Yang Xiaoyan;Li Sheng;Zhang Qi;Jin Juan;Yang Jianguo;Xiong Haoming;Dai Ruixia(Specialized Laboratory of Yersinia Pestis,Qinghai Institute for Endemic Disease Prevention and Control,Xining 810021,China;Department of Plague,Qinghai Institute for Endemic Disease Prevention and Control,Xining 810021,China)
出处
《中华地方病学杂志》
CAS
北大核心
2021年第11期880-883,共4页
Chinese Journal of Endemiology
基金
青海省科技厅基础研究计划项目(2019-ZJ-7074)
国家卫生健康委鼠疫防治研究重点实验室(共建)(2019PT310004)
青海省鼠疫防控及研究重点实验室(2021-ZJ-Y15)。
