摘要
采用顶空固相微萃取-气相色谱质谱法测定不同等级菜籽油中的挥发性气味物质。以总峰面积和出峰数量为指标,采用单因素实验对顶空固相微萃取条件(萃取头、萃取温度、吸附时间、解吸时间)进行优化,并在最优条件下对一级、三级、四级菜籽油的挥发性气味物质进行鉴定。结果表明:检测菜籽油中挥发性气味物质的最优顶空固相微萃取条件为采用50/30μm CAR/DVB/PDMS萃取头、萃取温度75℃、吸附时间30 min、解吸时间2 min,在此条件下经过GC-MS检测出的菜籽油挥发性气味物质成分最多,总峰面积最大。在最优萃取条件下测得一级、三级、四级菜籽油中挥发性气味物质分别有11、17、25种,其中一级菜籽油的主要挥发性气味物质为醛类,占57.94%;三级、四级菜籽油的主要挥发性气味物质为硫甙降解产物和杂环类物质,分别占37.33%、50.52%和17.50%、25.90%。
Headspace solid phase microextraction-gas chromatography-mass spectrometry was used to determine the volatile odor substances in different grades of rapeseed oils.The headspace solid phase microextraction conditions(extraction head,extraction temperature,adsorption time,and desorption time)were optimized by single factor experiment using total peak area and number of peaks as indicators.Under the optimal conditions,the volatile odor substances in the first,third and fourth grade rapeseed oils were identified.The results showed that the optimal headspace solid phase microextraction conditions were obtained as follows:with 50/30μm CAR/DVB/PDMS as extraction head,adsorption time 30 min,extraction temperature 75℃,and desorption time 2 min.Under the optimal extraction conditions,the most volatile odor substances of rapeseed oil were detected by GC-MS with the largest total peak area,and 11,17 and 25 volatile flavor components were measured in the first,third and fourth grade rapeseed oils,respectively.The main volatile odor substances of the first grade rapeseed oil were aldehydes,accounting for 57.94%;the main volatile odor substances of the third and fourth grade rapeseed oils were thioside degradation products and heterocyclic substances,accounting for 37.33%,50.52%and 17.50%,25.90%,respectively.
作者
周易枚
丁红梅
陈丹丹
程晓宏
杨俊
慕妮
刘尧刚
ZHOU Yimei;DING Hongmei;CHEN Dandan;CHENG Xiaohong;YANG Jun;MU Ni;LIU Yaogang(Nantong Food and Drug Inspection Center,Nantong 226006,Jiangsu,China;Tongzhou District Comprehensive Inspection and Testing Center of Nantong City,Nantong 226300,Jiangsu,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第11期40-43,72,共5页
China Oils and Fats
基金
南通市市级社会民生科技项目(MS12021027)
南通市市级科技计划(JCZ18030)。
关键词
菜籽油
挥发性气味物质
固相微萃取
rapeseed oil
volatile odor substance
solid phase microextraction
