摘要
目的以王不留行Vaccariae Semen为碳源,从中发现了王不留行炭纳米类成分(Vaccariae Semen Carbonisatum nano-components,VSC-NCs),并评价其对小鼠酒精性肝损伤的保护作用。方法利用马弗炉高温煅烧出王不留行炭,并从中提取分离出VSC-NCs。利用透射电子显微镜、紫外-可见分光光谱、傅里叶变换红外光谱(FT-IR)、荧光光谱、X射线光电子能谱(XPS)分析对其进行表征鉴定。采用小鼠酒精性肝损伤模型,对肝脏进行苏木精-伊红(hematoxylin-eosin,HE)染色,同时检测谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、总胆汁酸(total bile acid,TBA)的含量以及肝匀浆液中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛的水平来评价VSC-NCs的保肝作用。结果表征分析结果显示VSC-NCs为类球型,粒径分布在1.20~5.10 nm,晶格间距为0.23 nm,其主要由C、N、O 3种元素组成,表面含有羰基、羟基等基团。动物实验结果表明VSC-NCs可以显著降低酒精诱导的肝损伤小鼠ALT、AST、TBA和ALP含量,同时显著提高SOD水平和降低丙二醛的水平,表明VSC-NCs可以提高机体清除氧自由基的能力,减少脂质过氧化物的产生,从而达到保肝作用。结论首次从王不留行炭中发现了VSC-NCs,并证实了其对酒精所造成的肝损伤有明显保护作用,这不仅表明了VSC-NCs可以作为治疗酒精性肝损伤的一种潜在药物,也为研究中药物质基础提供了一种全新思维方式。
Objective Nano-components were discovered from Vaccaria Semen Carbonisatum(VSC)and explored their hepatoprotective effect on alcoholic liver injury in mice.Methods The VSC was calcinated by muffle furnace at a high temperature.By filtration,extraction and dialysis of the decoction,a new water-soluble substance was obtained and named as VSC nano-components(VSC-NCs).The VSC-NCs were characterized by using transmission electron microscopy(TEM),UV-Vis spectroscopy(UV-Vis),Fourier transform infrared spectroscopy(FT-IR),fluorescence spectroscopy,X-are ray photoelectron spectroscopy(XPS)and other instruments and methods.According to the alcoholic liver injury model,performing hematoxylin-eosin(HE)staining on the liver and measuring alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),total bile acid(TBA)and the levels of superoxide dismutase(SOD),malondialdehyde(MDA)in liver homogenate are to evaluate protective effect on liver injury of VSC-NCs.Results Characterization results showed that VSC-NCs were spherical,uniformly distributed in particle size between 1.20-5.10 nm,with a lattice spacing of 0.23 nm.The VSC-NCs were mainly composed by C,N,O and the surface contained carbonyl and hydroxyl groups.The animal experiment results showed that VSC-NCs significantly reduced the content of ALT,AST,TBA and ALP in mice with alcohol-induced liver injury,and significantly increased the level of SOD and reduced the level of MDA.The results indicated that VSC-NCs can improve the body's ability to scavenge oxygen free radicals and reduce the production of lipid peroxides,thereby protecting liver cells.Conclusion The VSC-NCs were discovered from Vaccaria Semen Carbonisatum for the first time and proved that they have a significant protective effect on liver damage caused by alcohol.This not only shows that VSC-NCs can be used as a potential drug for the treatment of alcoholic liver injury,but also provides a new way of thinking for the qualitative basis of traditional Chinese medicine in research.
作者
赵亚芳
李郁茹
陈玉民
赵玉升
罗娟
张一凡
孔慧
赵琰
屈会化
ZHAO Ya-fang;LI Yu-ru;CHEN Yu-min;ZHAO Yu-sheng;LUO Juan;ZHANG Yi-fan;KONG Hui;ZHAO Yan;QU Hui-hua(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Chinese Medicine Research,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第22期6825-6833,共9页
Chinese Traditional and Herbal Drugs
基金
中央高校基本科研业务费专项基金资助项目(2019-JYB-TD-001)。
关键词
王不留行炭
纳米类成分
酒精性肝损伤
保肝作用
谷丙转氨酶
谷草转氨酶
碱性磷酸酶
总胆汁酸
超氧化物歧化酶
丙二醛
Vaccaria Semen Carbonisatum
nano-components
alcoholic liver injury
hepatoprotective effect
alanine aminotransferase
aspartate aminotransferase
alkaline phosphatase
total bile acid
superoxide dismutase
malondialdehyde
