摘要
为降低酸奶在运输及储藏期间的后酸化,采用常压室温等离子体(atmospheric and room temperature plasma,ARTP)和新霉素诱变筛选,通过致死率曲线与阳性筛选率曲线确定最佳处理条件为ARTP处理30 s,新霉素筛选质量浓度为300 mg/mL,该条件下共获得7株生长特性良好,具有较低H;-ATP酶活性的副干酪乳杆菌(Lactobacillus paracasei)突变株。通过全脂牛乳发酵,突变菌株BJT-7在20 d储藏期间酸度维持在70°T以下,遗传稳定性良好。结果表明,ARTP是一种高效诱变方法,结合新霉素筛选可得到抗后酸化能力强的乳酸菌株。
In order to reduce the post-acidification problem of yogurt during transportation and storage,atmospheric and room temperature plasma(ARTP)and neomycin mutagenesis were used.According to the lethality curve and the positive screening rate curve,the best treatment conditions were determined as follows:ARTP irradiation time 30 s,neomycin screening concentration was 300 mg/mL.Seven mutant strains of Lactobacillus paracasei with good growth characteristics and low H;-ATPase activity were obtained.Through the fermentation of whole milk,the acidity of the mutant strain BJT-7 was maintained below 70°T during the 20 d of storage,and the genetic stability was good.The results showed that ARTP was an efficient mutagenesis method,combined with neomycin screening can obtain lactic acid strains with strong resistance to post-acidification.
作者
周虹瑾
霍向东
赵丹
林青
娄恺
袁华伟
ZHOU Hongjin;HUO Xiangdong;ZHAO Dan;LIN Qing;LOU Kai;YUAN Huawei(College of Life Science and Technology,Xinjiang University,Urumqi 830000,China;Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences/Xinjiang Laboratory of Special Environmental Microbiology,Urumqi 830091,China;Facuity of quality management and inspection quarantine/Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin University,Yibin 644000,Sichuan)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第23期112-117,共6页
Food and Fermentation Industries
基金
兵团重点研发计划专项(2020AB013)
固态发酵资源利用四川省重点实验室开放基金项目(2018GTJ014)
宜宾学院科研项目(2020YY04)。
关键词
抗后酸化
常压室温等离子体诱变
副干酪乳杆菌
新霉素
anti-postacidification
atmospheric and room temperature plasma mutagenesis
Lactobacillus paracasei
neomycin
