摘要
为研究酸化、低氧对大黄鱼(Larimichthys crocea)非特异性免疫及抗氧化能力的影响,设置4个处理组:对照组(pH 8.1,溶解氧DO 7.0 mg·L^(-1))、低氧组(pH 8.1,DO 3.5 mg·L^(-1))、酸化组(pH 7.35,DO 7.0 mg·L^(-1))及酸化-低氧组(pH 7.35,DO 3.5 mg·L^(-1))。于急性胁迫时间点(0、3、6、12、24、48、96 h)与慢性胁迫时间点(7、15、30 d)进行样品采集(n=6),检测其血清溶菌酶(LZM)、肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)活力的变化。结果显示,急性胁迫下,酸化组、酸化-低氧组及低氧组的肝脏SOD含量、MDA含量均呈现先升高后降低的趋势;酸化组、酸化-低氧组的血清LZM活力呈现先升高后降低的趋势;低氧组肝脏CAT活力、血清LZM活力则呈现先降低后升高的趋势。慢性胁迫下,酸化组中仅肝脏SOD含量与MDA含量、低氧组中仅肝脏SOD含量与CAT活力在胁迫后期呈现恢复至起始水平的趋势;酸化-低氧组中仅血清LZM活力、肝脏CAT活力与MDA含量在胁迫后期呈现恢复至起始水平的趋势。研究表明,在酸化与低氧环境中,尽管大黄鱼存在一定的自我调节机制,然而不管是单一因子的酸化、低氧还是酸化-低氧交互作用,均对大黄鱼的非特异性免疫及抗氧化能力存在一定程度的胁迫影响。
Large yellow croaker(Larimichthys crocea)is the main economic fish in the East China Sea,which occupies the third place in production of mariculture fish,and its production has showed an increasing trend in resent years.With the development of industry and commerce,the ocean absorbs excessive carbon dioxide from the atmosphere,which leads to the decline of ocean pH.Research shows that the increase of CO2 solubility in seawater will lead to the decrease of dissolved oxygen,so marine organisms may face the double stress of acidification and hypoxia.This will have a huge impact on the mariculture industry.To study the effects of acidification and hypoxia on nonspecific immunity and antioxidant capacity of Larimichthys crocea,four treatment groups were set in this experiment,which were control group(pH 8.1,DO 7.0 mg·L^(-1)),hypoxia group(pH 8.1,DO 3.5 mg·L^(-1)),acidification group(pH 7.35,DO 7.0 mg·L^(-1))and acidification-hypoxia group(pH 7.35,DO 3.5 mg·L^(-1)).L.crocea[(75.6±4.9)g]samples were collected with acute stress time(0,3,6,12,24,48,96 h)and chronic stress time(7,15,30 d)respectively(n=6).The activity of serum lysozyme(LZM)and liver catalase(CAT)and content of liver superoxide dismutase(SOD)and liver malondialdehyde(MDA)were detected.Under acute stress,results showed that SOD content and MDA content in liver of acidification group,hypoxia group and acidification-hypoxia group all showed a trend of first increasing and then decreasing;serum LZM activity in acidification group and acidification-hypoxia group increased first and then decreased,liver CAT showed a trend of increasing first,then decreasing and then increasing in the acidification group,while liver CAT activity and serum LZM activity in the hypoxia group showed a trend of first decreasing and then increasing.Under chronic stress,SOD content and MDA content in liver in acidification group,SOD content and CAT activity in liver in hypoxia group showed a tendency to recover to the initial level at the later stage of stress.In the acidification-hypoxic group,only serum LZM activity,liver CAT activity and MDA content tended to recover to the initial level at the later stage of stress.The peak values of serum LZM activity,liver SOD content,liver CAT activity and liver MDA content appeared in the acidification-hypoxia group at 48 h,acidification-hypoxia group on 30 d,hypoxia group at 12 h and hypoxia group on 30 d,respectively.This experiment data showed that,although L.crocea had a certain self-regulatory mechanism in the acidification and hypoxia environments,no matter it was the single factor of acidification,hypoxia or the interaction of acidification and hypoxia,there was a certain degree of stress effect on the non-specific immunity and antioxidant capacity of L.crocea.
作者
曾姣
陈润
王翠华
彭士明
王倩
郑亮
马凌波
ZENG Jiao;CHEN Run;WANG Cuihua;PENG Shiming;WANG Qian;ZHENG Liang;MA Lingbo(College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China)
出处
《海洋渔业》
CSCD
北大核心
2021年第6期680-692,共13页
Marine Fisheries
基金
国家重点研发计划项目(2018YFD0900603)
中国水产科学研究院基本科研业务费(2020XT10)。
关键词
酸化
低氧
大黄鱼
抗氧化酶
非特异性免疫
acidification
hypoxia
Larimichthys crocea
antioxidant enzymes
nonspecific immunity
