摘要
为提高米曲霉产氨肽酶的活性,对米曲霉CICC2066进行常压室温等离子体(ARTP)诱变处理,通过多轮筛选之后获得一株高产氨肽酶的米曲霉菌株M_(5),氨肽酶活性为241.2 U/mL,较出发的CICC2066提高了1.21倍。经过5次传代培养之后,产酶性能稳定,表明ARTP技术能够有效地提高米曲霉产氨肽酶的活性。
In order to improve the activity of aminopeptinase production by Aspergillus oryzae,Aspergillus oryzae CICC2066 is treated with atmospheric and room temperature plasma(ARTP)mutation theatment.After several rounds of screening,a strain M_(5) with high aminopeptinase productivity is obtained.The aminopeptinase activity is 241.2 U/mL,which is 1.21 times higher than that of CICC2066.After 5 times of subculture,the enzyme productivity is stable.The results show that ARTP technology could effectively improve the activity of aminopeptidase production by Aspergillus oryzae.
作者
张艳芳
孟广超
王选年
ZHANG Yan-fang;MENG Guang-chao;WANG Xuan-nian(Biotechnology Research Center of Xinxiang University,Xinxiang 453003,China;School of Life Sciences,Zhengzhou University,Zhengzhou 450001,China)
出处
《中国调味品》
CAS
北大核心
2021年第12期31-34,41,共5页
China Condiment
基金
新乡学院科技创新团队支撑项目(XXUTD20170106)。
关键词
ARTP
米曲霉
氨肽酶
高产菌株
ARTP
Aspergillus oryzae
aminopeptidase
high-yield strain
