摘要
为探究不同海拔下植烟土壤细菌群落结构特征及影响细菌群落结构的主要因素,在贵州省遵义地区选择海拔800(ZL)、1000(ZS)和1200m(ZH)的3块植烟土壤,采用16S rRNA通量测序法对植烟土壤微生物多样性进行分析。结果表明,ZL和ZH处理植烟土壤细菌α多样性均低于ZS处理;3个海拔下植烟土壤优势门为放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteriota),放线菌门的相对丰度以ZL处理最,较ZH处理提了34.22%;变形菌门表现则相反,ZH处理变形菌门丰度较ZL处理显著提。绿弯菌门与酸杆菌门丰度在3个海拔处理中表现较为一致,均为ZS>ZL>ZH。聚类热图分析结果显示,土壤含水率和pH与Armatimonadota和蓝细菌门(Gyanobacteria)存在显著正相关关系,与变形菌门存在显著负相关关系;变形菌门、厚壁菌门(Firmicutes)和菌门(Patescibacteria)与土壤微生物量氮存在显著正相关关系,而与速效钾存在极显著负相关关系。
In order to explore the characteristics and the main influencing factors of bacterial community structure of tobacco planting soil at different altitudes, three tobacco planting soils at different altitudes of 800(ZL), 1000(ZS) and 1200 m(ZH) were selected in Zunyi, Guizhou, and the bacterial community of tobacco planting soil was analyzed by 16 S rRNA high-throughput sequencing. The results showed that bacteria in tobacco planting soil in ZL and ZH treatments α-diversity were lower than that of ZS treatment. The dominant bacteria phyla in tobacco growing soil at three altitudes were Actinobacteria, Proteobacteria, Chloroflexi and Acidobacteria. The relative abundance of Actinobacteria was the highest in ZL(34.22%), which was higher than that in ZH;on the contrary,the abundance of Proteobacteria in ZH was significantly higher than that in ZL. The abundance of Chloroflexi and Acidobacteria were consistent at the three altitudes, which were ZS > ZL > ZH. The results of cluster heat map analysis showed that soil water content and pH value had a significant positive correlation with Armimonadota and Cyanobacteria, and a significant negative correlation with Proteobacteria;Proteobacteria, Firmicutes and Patescibacteria were significantly correlated with microbial biomass N, but negatively correlated with the content of available K.
作者
李茂森
高卫锴
任天宝
蒋石香
何小亚
骆乐琴
云菲
柯晓婷
Li Maosen;Gao Weikai;Ren Tianbao;Jiang Shixiang;He Xiaoya;Luo Leqin;Yun Fei;Ke Xiaoting(Tobacco college of Henan Agricultural University/Henan Engineering Research Center for Biochar/Henan Engineering Laboratory of Biochar Technology,Zhengzhou 450002,Henan,China;China Tobacco Guangdong Industrial Co.,Ltd.,Guangzhou 510032,Guangdong,China;Guizhou Provincial Tobacco Company Zunyi Branch,Zunyi 563000,Guizhou,China)
出处
《作物杂志》
北大核心
2021年第6期193-198,共6页
Crops
基金
国家重点研发计划(2017YFD0200808)
广东中烟工业有限责任公司科技攻关项目(2020440000340029)
河南青年干教师资助项目(2020GGJS047)。
关键词
植烟土壤
海拔
细菌群落
环境因子
Tobacco-planting soil
Altitude
Bacterial community
Environmental factors
