摘要
为研究不同耐盐性燕麦(Avena sativa)对盐胁迫的生理响应,以青永久195(耐盐材料)和709(盐敏感材料)为试验材料,采用营养液沙培法,对3周龄燕麦幼苗进行150 mmol·L^(−1) NaCl处理0(CK)、6、24、72 h,探讨了盐胁迫对两种燕麦幼苗光合作用、抗氧化特性、渗透调节物质含量的影响。结果表明:随着处理时间的延长,燕麦幼苗叶片的叶绿素含量、净光合速率(net photosynthetic rate,P_(n))、蒸腾速率(transpiration rate,T_(r))、气孔导度(stomatal conductance,G_(s))、胞间二氧化碳浓度(intercellular carbon dioxide concentration,C_(i))、K^(+)含量均有所下降;过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)活性、脯氨酸和可溶性糖含量呈先升高后降低的趋势,过氧化氢酶(catalase,CAT)活性、丙二醛(malondialdehyde,MDA)和Na^(+)含量均有所增加。其中在盐胁迫下耐盐材料青永久195叶片的叶绿素含量、P_(n)、T_(r)和G_(s)下降幅度小于盐敏感材料709;SOD、CAT、脯氨酸含量和可溶性糖含量均高于709,MDA含量低于709。盐胁迫6、24 h时,青永久195根中K^(+)、Na^(+)含量低于709,72 h时高于709;盐胁迫6和24 h时,青永久195叶中K^(+)含量高于709,Na^(+)含量低于709。综上,耐盐材料青永久195表现出较好的渗透调节能力,维持较高的抗氧化酶活性和生物膜稳定性。
This study aimed to investigate the physiological response of different salt-tolerant oats to salt stress,using Qingyongjiu 195(salt-tolerant)and 709(salt-sensitive)as test materials.Three-week-old oat seedlings grown in sand culture were treated with Hoagland nutrition containing 150 mmol·L^(−1) NaCl for 0(CK),6,24,and 72 h,and the effects of salt stress on photosynthesis,antioxidant properties,and osmotic regulation of the two oat seedlings were investigated.The results showed that the chlorophyll content,net photosynthetic rate(P_(n)),transpiration rate(T_(r)),stomatal conductance(G_(s)),intercellular carbon dioxide concentration(C_(i)),and K^(+)content of oat seedling leaves showed a decreasing trend with the extension of treatment time.The trend of peroxidase(POD),superoxide dismutase(SOD)activity,proline,and soluble sugar content first increased and then decreased,while other indices,including catalase(CAT)activity,malondialdehyde(MDA),and Na^(+)content showed an increasing trend.Under salt stress,the range of descent of chlorophyll content,P_(n),T_(r),and G_(s) in salt-tolerant Qingyongjiu 195 was less than that in salt-sensitive 709,whereas its SOD,CAT,proline content,and soluble sugar content were higher than that in 709,and MDA content was lower than that in 709.After salt stress at 6 and 24 h,the contents of K^(+)and Na^(+)in the roots of Qingyongjiu 195 were lower than that in 709,and higher than that in 709 at 72 h.After salt stress at 6 and 24 h,the content of K^(+)in the leaves of Qingyongjiu 195 was higher than in 709,whereas the content of Na^(+)was lower than that in 709.Overall,our study showed that salt-tolerant Qingyongjiu 195 possessed superior adjustment ability,antioxidant enzyme activity,and biofilm stability.
作者
王苗苗
赵桂琴
梁国玲
柴继宽
李娟宁
周向睿
WANG Miaomiao;ZHAO Guiqin;LIANG Guoling;CHAI Jikuan;LI Juanning;ZHOU Xiangrui(Pratacultural College,Gansu Agricultural University/Key Laboratory of Grassland Ecosystem,Ministry of Education/Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,Gansu,China;Academy of Animal and Veterinary Sciences of Qinghai University/Qinghai Academy of Animal Science and Veterinary Medicine/Key Laboratory of Use of Forage Germplasm Resources on Tibetan Plateau of Qinghai Provence,Xining 810016,Qinghai,China)
出处
《草业科学》
CAS
CSCD
北大核心
2021年第11期2200-2209,共10页
Pratacultural Science
基金
甘肃省科技重大专项计划(19ZD2NA002)
青海省青藏高原优良牧草种质资源利用重点实验室开放课题(2020-ZJ-Y03)
甘肃农业大学学科建设专项基金(GAU-XKJS-2018-010)。
关键词
燕麦
盐胁迫
光合作用
抗氧化特性
丙二醛含量
渗透调节
生理响应
oat
salt stress
photosynthesis
antioxidant properties
malondialdehyde content
osmotic regulation
physiological response
