摘要
以红景天为原料,分别考察超声提取法、乙醇回流提取法和乙醇浸提法对红景天苷含量的影响,以乙醇体积分数、浸提温度、超声时间以及料液比为考察因素,以红景天苷含量为考察指标,采用单因素试验结合响应面法分析与优化红景天苷提取条件。结果表明:提取效果为超声提取法(1.71±0.09)mg/g>乙醇浸提法(1.51±0.24)mg/g>乙醇回流提取法(1.47±0.05)mg/g,因此确定采用超声提取法结合乙醇浸提法提取红景天苷。乙醇体积分数60%、浸提温度30℃、超声时间2 h、料液比1∶80(g/mL)为最佳提取条件,在此条件下,提取液中红景天苷含量为2.35 mg/g。
Taking Rhodiola as raw material,the effects of ultrasonic extraction,ethanol reflux extraction and ethanol extraction on the content of salidroside were investigated respectively.Taking ethanol volume fraction,extraction temperature,ultrasonic time and solid-liquid ratio as investigation factors and salidroside content as investigation index,the extraction conditions of salidroside were analyzed and optimized by single factor test combined with response surface methodology.The results showed that the extraction effect was ultrasonic extraction(1.71±0.09)mg/g>ethanol extraction(1.51±0.24)mg/g>ethanol reflux extraction(1.47±0.05)mg/g.Therefore,ultrasonic extraction combined with ethanol extraction was selected to the extraction of salidroside.The optimum extraction condition was the ethanol volume fraction 60%,the extraction temperature 30℃,the ultrasonic time 2 h and the ratio of solid to liquid 1∶80(g/mL),under this condition,the content of salidroside in the extraction solution was 2.35 mg/g.
作者
郭娜
张宇婷
张颖智
乔巍
接伟光
GUO Na;ZHANG Yu-ting;ZHANG Ying-zhi;QIAO Wei;JIE Wei-guang(Department of Food and Environmental Engineering,East University of Heilongjiang,Harbin 150066,Heilongjiang,China)
出处
《粮食与油脂》
北大核心
2021年第12期103-107,共5页
Cereals & Oils
基金
黑龙江省自然科学基金联合引导项目(LH2021C076)
黑龙江东方学院横向科研课题(HDFKY190120)
2019年度联合乳品学院研究生导师开放基金项目(LHXYDS201902)
2021年度黑龙江东方学院校级科研创新团队建设项目(HDFKYTD202107)。
关键词
红景天苷
超声提取法
浸提法
回流提取法
响应面法
salidroside
ultrasonic extraction
extraction method
reflux extraction
response surface methodology
