摘要
目的:探讨F框蛋白2(F-box only protein 2,FBXO2)基因在人胃癌细胞系中表达及其对胃癌细胞增殖、迁移、侵袭和EMT的影响。方法:选择胃癌细胞系MGC-803、AGS、SGC-7901、MKN-28以及正常胃黏膜上皮细胞株GES-1,qPCR法检测细胞中FBXO2 mRNA表达水平。设计靶向抑制FBXO2表达的特异siRNA,并瞬时转染MGC-803细胞,转染siRNA无义序列的为阴性对照。qPCR法检测转染48 h后MGC-803细胞中FBXO2 mRNA表达水平;用MTT法、细胞划痕愈合法、Transwell小室法检测降低FBXO2表达对细胞增殖、迁移和侵袭的影响,WB法检测细胞中EMT相关蛋白E-cadherin、N-cadherin、vimentin的表达。结果:4种胃癌细胞中FBXO2 mRNA表达水平显著高于胃黏膜上皮细胞GES-1(P<0.05或P<0.01)。与阴性对照组相比,siRNAFBXO2组MGC-803细胞中FBXO2 mRNA表达下调(P<0.01),该细胞的增殖、迁移和侵袭能力受到显著抑制(P<0.05或P<0.01),E-cadherin蛋白表达明显升高(P<0.01),N-cadherin、vimentin蛋白表达显著降低(均P<0.01)。结论:低表达的FBXO2可抑制胃癌细胞的增殖、迁移和侵袭能力,该抑制作用可能与EMT过程有关。
Objective:To explore the expression of F-box only protein 2(FBXO2)gene in human gastric cancer cell lines,and its effect on the proliferation,migration,invasion and epithelial-mesenchymal transition(EMT)of gastric cancer cells.Methods:Gastric cancer cell lines(MGC-803,AGS,SGC-7901,MKN-28)and normal gastric epithelial cell line GES-1 were selected for this study;and Real-time quantitative PCR(qPCR)was used to detected the expression level of FBXO2 mRNA in the five cell lines.Designed a specific siRNA to inhibit the expression of FBXO2 and transiently transfected into MGC-803 call,and set up a negative control group for transfection of siRNA nonsense sequences.qPCR was used to detect the expression level of FBXO2 mRNA in MGC-803 cells 48 h after transfection;MTT,wound scratch,and Transwell assays were used to detect the effects of FBXO2 down-regulation on cell proliferation,migration and invasion,and WB was used to detect the expression of EMT-related proteins E-cadherin,N-cadherin and vimentin in cells.Results:The expression level of FBXO2 mRNA in the four gastric cancer cell lines was significantly higher than that in gastric epithelial cell line GES-1(P<0.05 or P<0.01).Compared with the negative control group,FBXO2 mRNA expression in the siRNA-FBXO2 group was decreased(P<0.01),and the proliferation,migration and invasion of the cells were significantly inhibited(P<0.05 or P<0.01),and E-cadherin protein expression was significantly increased(P<0.01),while N-cadherin and vimentin protein expression were significantly decreased(all P<0.01).Conclusion:The low expression of FBXO2 can efficiently inhibited the proliferation,migration and invasion of gastric cancer cells,which might be related with EMT.
作者
孙旭
朱军
李鑫
冯彩云
徐玲
赵琛健
章壮军
茆玲
SUN Xu;ZHU Jun;LI Xin;FENG Caiyun;XU Ling;ZHAO Chenjian;ZHANG Zhuangjun;MAO Ling(Clinical Pharmacy,Nanjing LuHe People's Hospital,Nanjing 211159,Jiangsu,China;Clinical Pharmacy,the First Hospital Affiliated to China Pharmaceutical University,Nanjing 210029,Jiangsu,China;Clinical Pharmacy,LuHe Branch of Nanjing Drum Tower Hospital,Nanjing 211159,Jiangsu,China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2021年第10期985-989,共5页
Chinese Journal of Cancer Biotherapy
基金
江苏省药学会-奥赛康医院药学基金资助项目(No.A201946)。
关键词
F框蛋白2
胃癌
增殖
迁移
侵袭
上皮-间质转化
F-box only protein 2(FBXO2)
gastric cancer
proliferation
migration
invasion
epithelial-mesenchymal transition(EMT)
