摘要
目的评价不同麻醉药对睡眠剥夺大鼠脑脊液β淀粉样蛋白(Aβ)和tau蛋白浓度的影响。方法SPF级健康雄性SD大鼠40只,3~4月龄,体重230~280 g,采用随机数字表法分为5组(n=8):对照组(C组)、睡眠剥夺组(SD组)、丙泊酚组(P组)、右美托咪定组(D组)和氯胺酮组(K组)。采用改良的多平台睡眠剥夺模型。P组、D组和K组睡眠剥夺72 h时分别腹腔注射丙泊酚100 mg/kg、右美托咪定100 mg/kg、氯胺酮80 mg/kg维持麻醉3 h,C组进入大平台自由活动72 h。于麻醉3 h时取脑脊液,采用ELISA法测定Aβ和tau蛋白浓度。结果与C组比较,SD组、P组、K组和D组大鼠脑脊液Aβ和tau蛋白浓度升高(P<0.05);与SD组比较,P组和K组大鼠脑脊液Aβ和tau蛋白浓度升高,D组大鼠脑脊液Aβ和tau蛋白浓度降低(P<0.05);与P组和K组比较,D组大鼠脑脊液Aβ和tau蛋白浓度降低(P<0.05)。结论右美托咪定可降低睡眠剥夺大鼠脑脊液Aβ和tau蛋白浓度,而丙泊酚和氯胺酮作用相反。
Objective To evaluate the effects of propofol,dexmedetomidine and ketamine on oncentrations ofβ-amyloid peptide(Aβ)and tau in cerebrospinal fluid(CSF)of sleep-deprived rats.Methods Forty SPF healthy male Sprague-Dawley rats,aged 3-4 months,weighing 230-280 g,were divided into 5 groups(n=8 each)using a random number table method:control group(group C),sleep deprivation group(SD),propofol group(group P),dexmedetomidine group(group D)and ketamine group(group K).The sleep deprivation was induced using the improved multi-platform sleep deprivation model.Propofol 100 mg/kg,dexmedetomidine 100 mg/kg and ketamine 80 mg/kg were intraperitoneally injected at 72 h of sleep deprivation to maintain anesthesia for 3 h in P,D and K groups,respectively.Group C entered the large platform for 72 h free activity.The CSF was collected at 3 h of anesthesia for measurement of concentrations of Aβand tau protein by enzyme-linked immunosorbent assay.Results The concentrations of Aβand tau protein in CSF were significantly higher in SD,P,K and D groups than in group C(P<0.05).Compared with group SD,the concentrations of Aβand tau protein in CSF were significantly increased in P and K groups,and the concentrations of Aβand tau protein in CSF were significantly decreased in group D(P<0.05).Conclusion Dexmedetomidine can decrease the the concentrations of Aβand tau protein in CSF of sleep deprived rats,while propofol and ketamine lead to the opposite effect.
作者
焦雅丽
解雅英
于建设
Jiao Yali;Xie Yaying;Yu Jianshe(Department of Anesthesiology,Inner Mongolia Maternity and Child Health Care Hospital,Hohhot 010010,China;Department of Anesthesiology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010010,China)
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2021年第10期1218-1221,共4页
Chinese Journal of Anesthesiology
