摘要
为了了解四川省成都地区猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus, PRRSV)流行毒株的分子生物学特性及遗传变异情况,试验采用生物信息学软件,对从四川省成都地区部分中小型养殖场猪群中收集的9份PRRSV阳性样品进行ORF5基因序列比对及遗传变异分析。结果表明:PCR扩增得到大小约为937 bp的条带,阳性克隆菌的测序结果与GenBank中PRRSV的同源性均大于98%;9株毒株SC20-13、SC20-19、SC20-27、SC20-31、SC20-35、SC20-76、SC20-81、SC20-95、SC21-11的ORF5基因核苷酸序列的同源性为84.4%~100%,其推导氨基酸序列的同源性为83.9%~100%。9株毒株均为PRRSV-2型,其中8株与谱系8的JXA1、CH-1a和CH-1R亲缘关系最近,而1株(SC21-11株)与谱系1的代表株NADC30的亲缘关系最近,SC21-11株GP5蛋白的第33位氨基酸缺失(S/N/G^(33))。说明PRRSV毒株变异频繁,GP5蛋白S/N/G^(33)缺失模式可以演化成为一类新型ORF基因缺失NADC30-like毒株。
In order to understand the molecular and genetic variation of Porcine reproductive and respiratory syndrome virus(PRRSV) in Chengdu area of Sichuan Province, the experiment used bioinformatics software to perform ORF5 gene sequence alignment and genetic variation analysis on nine PRRSV positive samples collected from pig herds of some small and medium-sized farms in Chengdu area of Sichuan Province. The results showed that a band with a size of about 937 bp was obtained by PCR amplification, and the sequencing results of the positive clones were more than 98% homologous to the PRRSV in GenBank. The homology of the ORF5 gene nucleotide sequences of nine sample strains SC20-13, SC20-19, SC20-27, SC20-31, SC20-35, SC20-76, SC20-81, SC20-95, SC21-11 was 84.4%-100%, and the homology of the deduced amino acid sequences was 83.9%-100%. The nine strains were all PRRSV-2 strains, of which eight strains were closely related to JXA1, CH-1 a and CH-1 R, the representative strain of lineage 8, and one strain(SC21-11) was closely related to NADC30, the representative strain of lineage 1. The 33 th amino acid of GP5 protein of the SC21-11 strain had been missing(S/N/G;). The results suggested that PRRSV strains frequently mutated, and the GP5 protein S/N/G;deletion pattern could evolve into a new type of NADC30-like strain with ORF gene deletion.
作者
肖璐
周泷
张毅
于吉锋
谢晶
林毅
李兴玉
叶勇刚
曹冶
潘梦
叶健强
吴学婧
康润敏
XIAO Lu;ZHOU Long;ZHANG Yi;YU Jifeng;XIE Jing;LIN Yi;LI Xingyu;YE Yonggang;CAO Ye;PAN Meng;YE Jianqiang;WU Xuejing;KANG Runmin(Sichuan Provincial Key Laboratory of Animal Breeding and Genetics,Sichuan Animal Science Academy,Chengdu 610066,China;College of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Sichuan Provincial Center for Animal Disease Control and Prevention,Chengdu 610041,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第23期11-17,共7页
Heilongjiang Animal Science And veterinary Medicine
基金
四川省省级科研院所基本科研业务费项目(SASA202001)
国家现代农业体系四川创新团队建设项目(Sccxtd-2021-08)
四川省财政运行专项(SASA2020CZYX006)
四川省农业科技成果转化项目(2020NZZJ011)。
关键词
猪繁殖与呼吸综合征
成都
ORF5
序列分析
基因缺失
Porcine reproductive and respiratory syndrome
Chengdu
ORF5
sequence analysis
gene deletion
