摘要
该研究以秀丽隐杆线虫(Caenorhabditis elegans,C.elegans)作为模式生物研究了黄花菜甲醇提取物对糖脂损伤的干涉作用。该研究利用UPLC-QQQ-MS/MS对黄花菜甲醇提取物中的成分进行了分析,并以野生型N2秀丽线虫为基础,以100μmol/mL蔗糖诱导糖损伤模型,以100μg/mL硬脂酸诱导脂损伤模型,对三个模型分别喂食0、400、800、1200μg/mL浓度的黄花菜甲醇提取物,检测黄花菜甲醇提取物对秀丽线虫产卵量、身长、寿命表型的干涉作用。结果表明,黄花菜甲醇提取物中的主要成分为芦丁,含量为5721.11 ng/mg。对普通N2秀丽线虫,喂食800μg/mL浓度黄花菜甲醇提取物的线虫比空白组线虫的总子代数和身长分别提高24.76%和7.73%;对糖损伤秀丽线虫,喂食800μg/mL浓度黄花菜甲醇提取物的线虫比空白组线虫的寿命和总子代数分别提高24.47%和3.40%;对于脂损伤秀丽线虫,喂食1200μg/mL浓度黄花菜甲醇提取物的线虫比空白组线虫的寿命、身长和总子代数分别提高30.04%、10.36%和97.32%。因此,中高浓度的黄花菜甲醇提取物对秀丽线虫的糖脂损伤有一定的干涉作用。
Caenorhabditis elegans(C.elegans)was used as a model organism to study the interference effects of methanol extract from Hemerocallis citrina Baroni on glycolipids damage.UPLC-QQQ-MS/MS was used to analyze the ingredients in methanol extract of Hemerocallis citrina Baroni,and based on the wild-type N2 C.elegans,sugar damage model was induced by 100μmol/mL sucrose,lipid injury model was induce by100μg/mL stearic acid.To observe the interference effects of methanol extract from Hemerocallis citrina Baroni,the three models were added with 0,400,800,1200μg/mL methanol extract of Hemerocallis citrina Baroni,egg production,body length and lifespan of C.elegans were detected.The results showed that the main component of methanol extract in Hemerocallis citrina Baroni was rutin with a content of 5721.11 ng/mg.For common N2 C.elegans,the total progeny and body length of the nematodes fed with 800μg/mL Hemerocallis citrina Baroni methanol extract increased by 24.76%and 7.73%,respectively,compared with the blank group;for sugar-damaged C.elegans,fed with 800μg/mL Hemerocallis citrina Baroni methanol extract,the lifespan and total progeny number of nematodes were increased by 24.47%and 3.40%respectively than those of the blank group;for lipid-damaged C.elegans,the lifespan,body length and total progeny number of nematodes fed with 1200μg/mL Hemerocallis citrina Baroni methanol extract were increased by 30.04%,10.36%and 97.32%than those of the blank group,respectively.Therefore,the results showed that the mid-to-high concentration of Hemerocallis citrina Baroni methanol extract had a certain interference effect on C.elegans glycolipids damage.
作者
秦喜悦
张雷
温艳斌
李景明
张雅丽
QIN Xiyue;ZHANG Lei;WEN Yanbin;LI Jingming;ZHANG Yali(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100089,China;Agriculture and Rural Bureau of Datong,Datong 037000,China)
出处
《现代食品科技》
CAS
北大核心
2021年第12期14-22,共9页
Modern Food Science and Technology
基金
山西省大同市农业农村局省校合作项目。
关键词
黄花菜甲醇提取物
秀丽线虫
糖脂损伤
Hemerocallis citrina Baroni methanol extract
Caenorhabditis elegans
glycolipids damage
