摘要
目的探讨TRIM38基因非CpG岛DNA甲基化与胶质瘤异柠檬酸脱氢酶(IDH)基因突变之间的关系。方法利用中国胶质瘤基因组图谱计划(CGGA)数据库的多组学数据和临床资料,比较在IDH野生型或突变型的胶质瘤中,TRIM38非CpG岛DNA甲基化的改变模式以及与基因表达和临床预后的关系。结果共纳入CGGA胶质瘤325例及非肿瘤对照脑组织(NTB组)11例,分析发现IDH野生型胶质瘤TRIM38非CpG岛DNA甲基化和基因表达,相对NTB组分别发生低甲基化(P=0.000)和高表达(P=0.007),且两者之间呈负相关(P=0.017)。生存分析显示,TRIM38非CpG岛DNA甲基化水平与IDH野生型肿瘤的预后有关(P=0.061)。结论IDH突变可能通过限制TRIM38基因非CpG岛DNA低甲基化介导的肿瘤促癌基因表达上调,为IDH突变相关的胶质瘤提供“保护作用”。
Objective To investigate the association between non-CpG island DNA methylation of TRIM38 and isocitrate dehydrogenase(IDH)gene mutations in glioma.Methods The multi-omics and clinical data from Chinese Glioma Genome Atlas(CGGA)were used to compare the differences in the non-CpG island DNA methylation pattern of TRIM38 and its association with gene expression and clinical prognosis between glioma with wild-type or mutant-type IDH.Results A total of 325 patients with glioma and 11 non-tumor brain(NTB)tissue samples from CGGA were included for analysis.It was found that compared with the NTB tissue samples,the glioma with wild-type IDH had a significant reduction in the non-CpG island DNA methylation of TRIM38(P=0.000)and a significant increase in the gene expression of TRIM38(P=0.007),with a significant negative correlation between them(P=0.017).The survival analysis showed that the non-CpG island DNA methylation level of TRIM38 was associated with the prognosis of glioma with wild-type IDH(P=0.061).Conclusions IDH mutations might exert a“protective effect”on gliomas associated with IDH mutation by inhibiting the upregulated expression of pro-oncogenic genes mediated by the non-CpG island DNA hypomethylation of TRIM38.
作者
胡维红
吴元明
殷安安
张静
HU Wei-Hong;WU Yuan-Ming;YIN An-An;ZHANG Jing(Medical College of Yanan University,Yan’an,Shaanxi 716000,China;Department of Biochemistry and Molecular Biology,Air Force Military Medical University,Xi’an,Shaanxi 710032,China;Department of Neurosurgery,Xijing Institute of Clinical Neuroscience,Xijing Hospital,Air Force Military Medical Universi-ty,Xi’an,Shaanxi 710032,China)
出处
《国际神经病学神经外科学杂志》
2021年第5期415-418,共4页
Journal of International Neurology and Neurosurgery
基金
中国博士后科学基金(2019M653971)
山东省自然科学基金青年项目(ZR2020QH233)。
