摘要
目的探讨与分析β-榄香烯对肺癌小鼠模型肿瘤生长及组织胰岛素样生长因子结合蛋白(IGFBP)和血管内皮生长因子(VEGF)表达的影响。方法选取36只清洁级BALB/c裸鼠建立Lewis肺癌模型,然后根据随机数字表法分为3组:模型组、顺铂组和顺铂+β-榄香烯组,每组各12只。其中,顺铂组给予腹腔注射顺铂0.2 m L;顺铂+β-榄香烯组依次给予0.2 m L顺铂和β-榄香烯腹腔注射,每隔2 d注射1次,持续7次21 d;模型组给予腹腔注射等剂量的0.9%氯化钠溶液。分别于治疗14 d和21 d后,采用游标卡尺测量肿瘤最长径和最短经,记录肿瘤的体积变化。治疗21 d后,剥离肿瘤组织称重并计算抑瘤率;取小鼠的脾和胸腺称重并计算抑瘤、脾指数和胸腺指数;取肿瘤组织,蛋白质印迹法检测肿瘤组织IGFBP1与VEGF的表达。结果治疗14~21 d后,顺铂组、顺铂+β-榄香烯组的肿瘤体积显著小于模型组,顺铂+β-榄香烯组也显著小于顺铂组,差异均有统计学意义(P<0.05)。治疗21 d后,顺铂组、顺铂+β-榄香烯组的抑瘤率显著高于模型组,瘤重显著小于模型组,顺铂+β-榄香烯组抑瘤率也显著高于顺铂组,瘤重显著小于模型组,差异均有统计学意义(P<0.05)。顺铂组、顺铂+β-榄香烯组治疗21 d后的脾脏指数和胸腺指数高于模型组,顺铂+β-榄香烯组高于顺铂组,差异均有统计学意义(P<0.05)。顺铂组、顺铂+β-榄香烯组治疗21 d后体内的肿瘤组织IGFBP1与VEGF蛋白相对表达水平低于模型组,顺铂+β-榄香烯组低于顺铂组,差异均有统计学意义(P<0.05)。结论β-榄香烯在肺癌小鼠模型的应用能抑制肺肿瘤生长并增强脾脏和胸腺功能,其作用机制可能与抑制IGFBP1与VEGF的表达有关。
Objective To investigate and analyze the effects ofβ-elemene on tumor growth and expression of insulin-1 ike growth factor binding protein(IGFBP1)and vascular endothelial growth factor(VEGF)in lung cancer mouse model.Methods Thirty-six clean BALB/c nude mice were selected to establish the Lewis lung cancer model,and then divided into three groups according to the random number table method:model group,cisplatin group and cisplatin+β-elemene group,each group 12 mice.Among them,the cisplatin group was given intraperitoneal injection of 0.2 m L of cisplatin;the cisplatin+β-elemene group was given intraperitoneal injection of 0.2 m L of cisplatin andβ-elemene sequentially,once every 2 d,for 7 times for 21 d;The model group was given the same dose of normal saline intraperitoneally.After 14 to 21 days of treatment,the longest diameter and shortest meridian of the tumor were measured with a vernier caliper,and the volume change of the tumor was recorded.After 21 days of treatment,,the tumor tissue was stripped and weighed and the tumor inhibition rate was calculated;Spleen and thymus of the mouse were taken to weigh and calculate the tumor inhibition,spleen index and thymus index;The tumor tissues were taken,and Western blotting method was used to detect the expression of IGFBP1 and VEGF in tumor tissues.Results After 14 to 21 days of treatment,the tumor volume of the cisplatin group and the cisplatin+β-elemene group was significantly smaller than that of the model group,and the cisplatin+β-elemene group was also significantly smaller than that of the cisplatin group,the differences were statistically significant(P<0.05).After 21 days of treatment,the tumor inhibition rate of the cisplatin group and the cisplatin+β-elemene group was significantly higher than that of the model group,and the tumor weight was significantly smaller than that of the model group,the differences were statistically significant(P<0.05).The tumor inhibition rate of the cisplatin+β-elemene group was also significantly higher than that of the cisplatin group,and the tumor weight was significantly smaller than that of the model group,the differences were statistically significant(P<0.05).The spleen index and thymus index of the cisplatin group and the cisplatin+β-elemene group were higher than the model group after 21 days of treatment,and the cisplatin+β-elemene group was higher than that of the cisplatin group,the differences were statistically significant(P<0.05).The relative expression levels of IGFBP1 and VEGF protein in the tumor tissues of the cisplatin group and cisplatin+β-elemene group were lower than that of the model group after 21 days of treatment,and the cisplatin+β-elemene group was lower than that of the cisplatin group,the differences were statistically significant(P<0.05).Conclusion The application ofβ-elemene in mouse models of lung cancer can inhibit the growth of lung tumors and enhance the function of spleen and thymus.Its mechanism may be related to the inhibition of the expression of IGFBP1 and VEGF.
作者
杨婧
赵鹏
刘冬
申炜炜
YANG Jing;ZHAO Peng;LIU Dong(Department of Oncology,The Second Affiliated Hospital of Air Force Military Medical University,Xi'an Shaanxi 710038,China)
出处
《临床和实验医学杂志》
2021年第23期2465-2468,共4页
Journal of Clinical and Experimental Medicine
基金
陕西省重点研发计划项目—一般项目(编号:2019SF-085)。
关键词
小鼠模型
肺癌
Β-榄香烯
转移侵袭
血管内皮生长因子
胰岛素样生长因子结合蛋白
Mice model
Lung cancer
β-elemene
Metastasis and invasion
Vascular endothelial growth factor
Insulin-like growth factor binding protein
