摘要
目的探讨PM 2.5对去势致骨质疏松症大鼠腰椎(L_(3~5))、右侧股骨骨密度及左侧股骨骨生物力学特性的影响。方法将3周龄雌性SD大鼠(SPF级)适应生长一周后随机分为假手术组、模型对照组和PM 2.5低、中、高剂量组,行气道滴注PM 2.5混悬液或生理盐水,滴注5个月后将模型对照组和PM 2.5各剂量组通过切除大鼠双侧卵巢建立绝经后骨质疏松症模型,假手术组仅切除卵巢周围约1 g的脂肪组织。继续气管滴注至第9个月末,其间分别测定去势后滴注第7、9个月末各组腰椎(L_(3~5))、右侧股骨骨密度(bone mineral density, BMD)数值,处死大鼠后取左侧股骨进行三点弯曲试验。结果气管滴注7个月后,各组大鼠腰椎(L_(3~5))及右侧股骨BMD比较差异无统计学意义(P>0.05)。滴注9个月后,PM 2.5各剂量组大鼠腰椎及右侧股骨BMD明显低于模型对照组,且PM 2.5各剂量组之间呈现一定的剂量关联趋势,中、高剂量组BMD降低趋势明显,差异有统计学意义(P<0.05)。此外,从骨生物力学角度分析,与假手术组相比,模型对照组、PM 2.5低剂量组的弹性载荷、极限载荷、弹性模量均下降,且差异有统计学意义(P<0.05);与模型对照组比较,PM 2.5各剂量组的股骨弹性载荷、极限载荷和弹性模量降低(P<0.05),下降趋势与BMD一致,高剂量组下降最为显著。结论 PM 2.5加速了去势大鼠的骨密度降低,造成骨生物力学破坏,与PMOP的严重程度相关,具体机制有待探讨。
Objective To investigate the effects of PM2.5 on bone mineral density(BMD) of lumbar vertebrae(L_(3~5)), right femur and biomechanical properties of left femur in ovariectomized rats with osteoporosis. Methods Three-week-old female SD rats were randomly divided into sham operation group, model control group and PM 2.5 low, middle and high dose groups after one week of adaptive growth. Postmenopausal osteoporosis model was established by bilateral ovariectomy in model control group and PM 2.5 dose group at the end of 5 months. Only 1 g adipose tissue around ovary was removed in sham operation group. The values of bone mineral density(bone mineral density, BMD) of lumbar vertebrae(L_(3~5)) and right femur in each group at the end of 7 and 9 months after castration were measured by tracheal instillation to the end of September, after the rats were killed, the left femur was taken for three-point bending test. Results At the end of 7 months after tracheal instillation, there was no significant difference in BMD of lumbar vertebrae(L_(3~5)) and right femur among all groups. At the end of 9 months, the BMD of lumbar vertebrae and right femur in PM 2.5 castrated dose group was significantly lower than that in sham operation group and model control group, and there was a certain dose correlation trend among PM 2.5 groups, and the BMD decreased significantly in middle and high dose groups. In addition, from the point of view of bone biomechanics, compared with the sham operation group, the elastic load, ultimate load and elastic modulus of the model control group and the low dose PM 2.5 group were lower than those of the sham operation group, and the elastic load, limit load and elastic modulus of the femur in the PM 2.5 dose group were lower than those in the model control group(P<0.05), the downward trend was consistent with the BMD data, and the decrease in the high dose group was the most significant. Conclusion PM 2.5 accelerates the decrease of bone mineral density and causes bone biomechanical destruction in PMOP rats, which is related to the severity of PMOP. The specific mechanism remains to be explored.
作者
倪清丽
黄惠娟
林妹珠
董怡君
刘宗玉
蔡芬
张小蕊
NI Qingli;HUANG Huijuan;LIN Meizhu;DONG Yijun;LIU Zongyu;CAI Fen;ZHANG Xiaorui(Department of Obstetrics and Gynecology,The 90th Hospital of Joint Logistic Support Force,Fuzhou 350025,China;East Hospital,Xiamen University,Fuzhou 350025,China;Fuzong Clinical Medical College,Fujian Medical University,Fuzhou 350025,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2021年第12期1726-1729,1744,共5页
Chinese Journal of Osteoporosis
基金
国家自然科学基金(81771547)。
关键词
绝经后骨质疏松
PM2.5
生物力学特性
骨密度
postmenopausal osteoporosis
PM 2.5
biomechanical properties
bone mineral density
