摘要
【目的】鉴定并分析CsETR基因,预测其潜在分子功能,以期从分子水平了解乙烯受体在茶树生长过程中应对胁迫的作用机制。【方法】采用生物信息学分析方法鉴定ETR基因家族成员并预测其潜在分子功能,采用实时荧光定量分析法分析其在不同胁迫处理下的表达水平。【结果】从茶树基因组中确定了6个茶树ETR基因家族成员,均包含N端跨膜区、GAF区和组氨酸(His)激酶结构域;系统发育树分析显示其为两组,与乙烯受体的两个亚家族分别对应;茶树ETR基因家族中每个成员含有1-12个外显子。ETR家族基因在茶树果实和茎中表达量较高,且该家族表现出明显的组织表达特异性。实时荧光定量分析结果显示:低温处理下,6个基因的表达水平上调,其中ERS1-1基因的表达水平上调最为明显;在植物生长调节剂ABA、JA和GA处理下大部分基因表达水平上调,其中ERS1-3基因上调最为明显,而ETR2-2基因在ABA处理下表达水平呈缓慢下调趋势。【结论】共鉴定出6个CsETR基因家族成员,并预测分析了其潜在的分子功能,该基因家族成员在果实和茎中表达量较高,且低温能诱导CsETR基因的表达,其中ERS1-3基因在ABA、MeJA和GA的处理下表达量显著上调。
【Objective】 CsETRs were identified and analyzed to predict the potential molecular functions involving the mechanism of ethylene receptor in response to stress in tea plants. 【Method】 Bioinformatics was used to identify members in the ETR family and predict their potential molecular functions. Real-time fluorescence quantitative analysis was employed for the expressions of the genes under stresses.【Result】 Six ETRs were identified from the genome of tea plants. They all consisted of N-terminal transmembrane region, GAF region, and histidine(His) kinase domain. Phylogenetic tree analysis divided them into two groups. Each member of the ETR family contained 1-12 exons. Significantly differentiated in tissues, the expressions of ETRs were high in the fruits and stems. The fluorescence quantitative expressions of the 6 genes, especially ERS1-1, were upregulated to varying degrees when exposed to low temperature. Under the stress of plant growth regulators ABA, JA, or GA, most of the genes were upregulated, especially ERS1-3, but ETR2-2 downregulated slowly when treated by ABA. 【Conclusion】 On tea plants, 6 CsETRs in the family were identified with their potential molecular functions predicted and analyzed in this study. CsETRs were highly expressed in the fruits and stems that could be induced by lowtemperature stress. Whereas ABA, MeJA, or GA could significantly upregulate the expression of ERS1-3.
作者
晏美红
郑玉成
侯炳豪
陈雪津
陈晓君
叶乃兴
YAN Meihong;ZHENG Yucheng;HOU Binghao;CHEN Xuejin;CHEN Xiaojun;YE Naixing(College of Horticulture,Fujian Agriculture and Forestry University/Key Laboratory of Tea Science at University in Fujian,Fuzhou,Fujian 350002,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2021年第10期1160-1168,共9页
Fujian Journal of Agricultural Sciences
基金
中国乌龙茶产业协同创新中心专项(闽教科[2015]75号)
安溪茶叶重大科技创新专项(AX2021001)
福建农林大学大学生创新创业训练计划项目(20190389154)
福建张天福茶叶发展基金会科技创新基金项目(FJZTF01)。
关键词
茶树
ETR基因
植物生长调节剂
非生物胁迫
表达分析
Camellia sinensis
ETR gene
plant growth regulators
abiotic stress
expression analysis
