摘要
抗坏血酸过氧化物酶(ascorbate peroxidase,APX)是活性氧清除系统的重要成员之一,会对细胞的结构以及功能产生危害。为了揭示HbAPXs基因家族中的6个成员位于巴西橡胶树的细胞核染色体上的具体位置,展现Hb APXs家族基因6个成员之间的分布情况。本研究采用荧光原位杂交技术(fluorescence in situ hybridization,FISH),对HbAPXs基因家族6个成员(HbAPX1,HbAPX3,HbAPX4,HbAPX5,HbAPX6,HbAPX7)进行了物理定位分析。实验结果表明:HbAPX1定位于4号染色体的短臂,其信号的位点到染色体的着丝粒之间的百分比距离为12.51;而HbAPX3、HbAPX4、HbAPX5、HbAPX6和HbAPX7定位在7、1、2、9和3号染色体上,均为长臂,其基因信号的位点到相对应的染色体的着丝粒之间的百分比距离分别为80.27、24.72、87.19、13.69和57.63,并讨论了其与已完成定位基因之间的位置关系。HbAPXs基因家族中的6个成员互为独立的基因,均分布在不同的染色体上。本研究通过物理定位确定这些基因在染色体上的具体位置和相互关系,可为橡胶树育种及基因组学的研究提供细胞遗传学依据。
Ascorbate peroxidase(APX) is one of the key members of reactive oxygen species scavenging system,which will harm the structure and function of cells.In order to reveal the actual position of 6 members of HbAPXs gene family of the Hevea brasiliensis on the nuclear chromosomes,and to show the distribution of these genes,we located six members of the Hb APXs gene family(HbAPX1,HbAPX3,HbAPX4,HbAPX5,HbAPX6,HbAPX7) on chromosomes of Hevea brasiliensis by fluorescence in situ hybridization(FISH).The results showed that HbAPX1 the short arm located on chromosome 4,the percentage distance between the site of the signal and the centromere of the chromosome was 12.51,while the HbAPX3,HbAPX4,HbAPX5,HbAPX6 and HbAPX7 located on chromosomes 7,1,2,9 and 3 were long arms,and the percentage distance between the site of the gene signal and the centromere of the corresponding chromosome were 80.27,24.72,87.19,13.69 and 57.63,respectively,and their positional relationship with other localized genes was also discussed.HbAPXs six me mbers of the gene family were independent genes,all distributed on different chromosomes.In this study,the location and relationship of these genes on chromosomes were determined by physical localization,which can provide cytogenetic basis for Hevea brasiliensis breeding and genomics research.
作者
刁艳茹
王宇翔
党一敏
王英
高和琼
庄南生
Diao Yanru;Wang Yuxiang;Dang Yimin;Wang Ying;Gao Heqiong;Zhuang Nansheng(College of Tropical Crops,Hainan University,Haikou,570228;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101)
出处
《分子植物育种》
CAS
北大核心
2021年第22期7391-7397,共7页
Molecular Plant Breeding
基金
国家自然科学基金项目(31760216,31360186)
国家天然橡胶产业技术体系项目(CARS-33-YZ2)
海南大学热带作物学院研究生科研创新课题(Hys2019-139)。
